Construct: ORF TRCN0000480872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017800.1_s317c1
- Derived from:
- ccsbBroadEn_13886
- DNA Barcode:
- TTGTGGGAATCAAATTGGTGGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNMB1 (3779)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3779 | KCNMB1 | potassium calcium-activated... | NM_004137.4 | 62.6% | 49.2% | (many diffs) |
| 2 | mouse | 16533 | Kcnmb1 | potassium large conductance... | NM_031169.4 | 54.5% | 44.1% | (many diffs) |
| 3 | mouse | 16533 | Kcnmb1 | potassium large conductance... | XM_006514541.3 | 54.5% | 44.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 480
- ORF length:
- 390
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTGAAGAA GCTGGTGATG GCCCAGAAGC 121 GGGGAGAGAC ACGAGCCCTT TGCCTGGGTG TAACCATGGT GGTGTGTGCC GTCATCACCT 181 ACTACATCCT GGTCACGACT GTGCTGCCCC TCTACCAGAA AAGCGTGTGG ACCCAGGAAT 241 CCAAGTGCCA CCTGATTGAG ACCAACATCA GGGACCAGGA GGAGCTGAAG GGCAAGAAGG 301 TGCCCCAGTA CCCATGCCTG TGGGTCAACG TGTCAGCTGC CGGCAGGTGG GCTGTGCTGT 361 ACCACACGGA GGACACTCGG GACCAGAACC AGCAGGTACT GAACTGGAGG GATGGGGACA 421 CATCCCTTTA TCCCTGTCAG GTGTGTGAGC CTGTTCCTAA CTGTCCCTGT CCCCCAGGCT 481 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGATTGTG GGAATCAAAT TGGTGGATCA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt