Construct: ORF TRCN0000480883
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013207.1_s317c1
- Derived from:
- ccsbBroadEn_06400
- DNA Barcode:
- CCTAAAGTTGCGACTCTAATAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AGFG2 (3268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480883
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | NM_006076.5 | 98.8% | 69.2% | 1022_1023insC;1046T>C;1429_1443del |
| 2 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_005250306.2 | 96.6% | 67.7% | (many diffs) |
| 3 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012112.1 | 80.7% | 51.8% | (many diffs) |
| 4 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012113.1 | 61.5% | 80% | 878_879delTC;883delT;885_886ins547 |
| 5 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012114.1 | 46.7% | 18.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1161
- ORF length:
- 1095
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gatggcggcg aagaagggcc cgggcccggg cggcggggtc agcgggggca 121 aggcggaggc ggaggcggcc tcggaggtgt ggtgccgtcg ggtgcgggag ctgggtggct 181 gcagccaggc cgggaaccgc cactgcttcg agtgcgccca gcgcggggtc acctacgtgg 241 atatcaccgt gggcagcttc gtgtgcacca cctgctccgg cctcctgaga gggctgaacc 301 cccctcatcg tgtcaagtca atctccatga caactttcac tgagcctgaa gtagtattcc 361 tgcaatcccg tggaaatgag gtttgccgga agatttggtt gggtctgttt gatgctcgga 421 catctttagt accagattcc agggatcctc agaaagtgaa ggagtttctc caggaaaaat 481 atgagaagaa gagatggtat gtccccccag accaagtcaa ggggcccact tataccaaag 541 gcagtgcctc cacccctgtg cagggctcca tcccagaagg gaagcccctt cggacacttc 601 tgggtgatcc tgcaccgtct ctctcagttg ctgcctccac ctcgagccag cccgtcagtc 661 agtctcacgc tcggacatcc caggcccgga gcactcagcc acctccccac tcctctgtca 721 aaaaagccag tactgacctg ctggctgaca tcggtggaga cccctttgct gcaccccaga 781 tggcaccagc ttttgctgca ttccctgcct ttgggggcca gacaccttcc caaggaggct 841 ttgccaactt tgatgccttt agcagtggcc ccagctcttc tgtgtttgga agcctccctc 901 cagctggtca agcctcgttc caggcccagc caactcctgc agggagcagc caggggactc 961 catttggtgc cactcccctg gcacccgcca gtcagccaaa cagcctcgca gacgtgggca 1021 gcttcctggg acccggggtg cccgctgcag gtgttcctag cagcctcTTC GGGATGGCTG 1081 GCCAGGTCCC CCCCGCTCCA GTCTGTCACG ACGGGCGGCG GCGGCGGCAG CAGCACAGGG 1141 CTGGCCTTTG GAGCCTTCAC TAACCCTTTC ACAGCTCCCG CCGCCCAGTC CCCGCTGCCT 1201 TCCACCAACC CGTTCCAGCC CAATGGCTTG GCGCCAGGGC CCGGCTTTGG GATGAGCAGT 1261 GCTGGGCCTG GCTTCCCCCA GGCAGTGCCA CCCACTGGGG CCTTTGCCAG CTCCTTCCCA 1321 GCACCGCTGT TCCCCCCGCA GACCCCGCTT GTTCAGCAGC AGAATGGCTC TTCCTTCGGG 1381 GACTTAGGAT CAGCCAAGTT GGGGCAGAGG CCACTGAGCC AGCCAGCTGG GATCTCCACC 1441 AACCCCTTCA TGACTGGACC CTCATCAAGC CCATTCGCCT CCAAACCTCC AACCACCAAC 1501 CCCTTCTTGT ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC 1561 CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG 1621 ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTAA AGTTGCGACT CTAATAAGTA 1681 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt