Construct: ORF TRCN0000480886
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012133.1_s317c1
- Derived from:
- ccsbBroadEn_08446
- DNA Barcode:
- GCCAGCTCACAAAAGCGTAGCCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTARC2 (54996)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480886
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54996 | MTARC2 | mitochondrial amidoxime red... | NM_001317338.2 | 99.7% | 99.7% | 72G>C;132G>T;528G>A |
| 2 | human | 54996 | MTARC2 | mitochondrial amidoxime red... | NM_017898.5 | 99.7% | 99.7% | 72G>C;132G>T;528G>A |
| 3 | human | 54996 | MTARC2 | mitochondrial amidoxime red... | NM_001331042.2 | 74.6% | 73.3% | (many diffs) |
| 4 | human | 54996 | MTARC2 | mitochondrial amidoxime red... | XM_011509684.1 | 72.9% | 72.8% | 0_1ins270;2T>G;258G>A |
| 5 | human | 54996 | MTARC2 | mitochondrial amidoxime red... | XR_247029.5 | 31% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1071
- ORF length:
- 1005
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cgcttccagc tcctccgcgc tggcccgcct cggcctccca gcccggccct 121 ggcccaggtg gctcggcgtc gccgcgctag gactggccgc cgtggccctg gggactgtcg 181 cctggcgccg cgcatgtccc aggcggcgcc ggcggctgca gcaggtgggc accgtggcga 241 agctctggat ctacccggtg aaatcctgca aaggggtgcc ggtgagcgag gctgagtgca 301 cggccatggg gctgcgcagc ggcaacctgc gggacaggtt ttggctggtg attaaggaag 361 atggacacat ggtcactgcc cgacaggagc ctcgcctcgt gctcatctcc atcatttatg 421 agaataactg cctgatcttc agggctccag acatggacca gctggttttg cctagcaagc 481 agccttcctc aaacaaactc cacaactgca ggatatttgg ccttgacatt aaaggcagag 541 actgtggcaa tgaggcagcT AAGTGGTTCA CCAACTTCTT GAAAACTGAA GCATATAGAT 601 TGGTTCAATT TGAGACAAAC ATGAAGGGAA GAACATCAAG AAAACTTCTC CCCACTCTTG 661 ATCAGAATTT CCAGGTGGCC TACCCAGACT ACTGCCCGCT CCTGATCATG ACAGATGCCT 721 CCCTGGTAGA TTTGAATACC AGGATGGAGA AGAAAATGAA AATGGAGAAT TTCAGGCCAA 781 ATATTGTGGT GACCGGCTGT GATGCTTTTG AGGAGGATAC CTGGGATGAA CTCCTAATTG 841 GTAGTGTAGA AGTGAAAAAG GTAATGGCAT GCCCCAGGTG TATTTTGACA ACGGTGGACC 901 CAGACACTGG AGTCATAGAC AGGAAACAGC CACTGGACAC CCTGAAGAGC TACCGCCTGT 961 GTGATCCTTC TGAGAGGGAA TTGTACAAGT TGTCTCCACT TTTTGGGATC TATTATTCAG 1021 TGGAAAAAAT TGGAAGCCTG AGAGTTGGTG ACCCTGTGTA TCGGATGGTG TACCCAACTT 1081 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1141 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1201 CTTGTGGAAA GGACGAGCCA GCTCACAAAA GCGTAGCCCG ACGCGTTAAG TCgacaatca 1261 acctctggat tacaaaattt gtgaaagatt