Construct: ORF TRCN0000480893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010201.1_s317c1
- Derived from:
- ccsbBroadEn_10431
- DNA Barcode:
- AACCGTCGTCTGCTGCTTGTGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HADHB (3032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | NM_000183.3 | 99.6% | 99.3% | 3_4insACT;625C>T;825T>C |
| 2 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | XM_011532803.1 | 99.6% | 99.3% | 3_4insACT;625C>T;825T>C |
| 3 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | NM_001281512.1 | 96.4% | 96.2% | (many diffs) |
| 4 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | XM_024452830.1 | 95.2% | 94.5% | (many diffs) |
| 5 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | NM_001281513.2 | 93.9% | 92.8% | (many diffs) |
| 6 | human | 3032 | HADHB | hydroxyacyl-CoA dehydrogena... | XM_024452831.1 | 93.9% | 92.8% | (many diffs) |
| 7 | mouse | 231086 | Hadhb | hydroxyacyl-Coenzyme A dehy... | NM_001289798.1 | 88.9% | 90.9% | (many diffs) |
| 8 | mouse | 231086 | Hadhb | hydroxyacyl-Coenzyme A dehy... | NM_001289799.1 | 88.9% | 90.9% | (many diffs) |
| 9 | mouse | 231086 | Hadhb | hydroxyacyl-Coenzyme A dehy... | NM_145558.2 | 88.9% | 90.9% | (many diffs) |
| 10 | mouse | 231086 | Hadhb | hydroxyacyl-Coenzyme A dehy... | XM_017320828.1 | 88.9% | 90.9% | (many diffs) |
| 11 | mouse | 231086 | Hadhb | hydroxyacyl-Coenzyme A dehy... | XM_017320829.1 | 88.9% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1491
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tactatcttg acttacccct ttaaaaatct tcccactgca tcaaaatggg 121 ccctcagatt ttccataaga cctctgagct gttcctccca gctacgagct gccccagctg 181 tccagaccaa aacgaagaag acgttagcca aacccaatat aaggaatgtt gtggtggtgg 241 atggtgttcg cactccattt ttgctgtctg gcacttcata taaagacctg atgccacatg 301 atttggctag agcagcgctt acgggtttgt tgcatcggac cagtgtccct aaggaagtag 361 ttgattatat catctttggt acagttattc aggaagtgaa aacaagcaat gtggctagag 421 aggctgccct tggagctggc ttctctgaca agactcctgc tcacactgtc accatggctt 481 gtatctctgc caaccaagcc atgaccacag gtgttggctt gattgcttct ggccagtgtg 541 atgtgatcgt ggcaggtggt gttgagttga tgtccgatgt ccctattcgt cactcaagga 601 aaatgagaaa actgatgctt gatctcaata aggccaaatc tatgggccag cgactgtctt 661 taatctctaa attccgattt aatttcctag catctgagct ccctgcggtt tctgagttct 721 ccaccagtga gaccatgggc cactctgcag accgactggc cgctgccttt gctgtttctc 781 ggctggaaca ggatgaatat gcactgcgct ctcacagtct agccaagaag gcacaggatg 841 aaggactcct ttctgatgtg gtacccttca aagtaccagg aaaagataca gtcaccaaag 901 ataatggcat ccgtccttcc tcactggagc agatggccaa actaaaacct gcattcatca 961 agccctacgg cacagtgaca gctgcaaatt cttctttctt gactgatggt gcaTCTGCAA 1021 TGTTAATCAT GGCGGAGGAA AAGGCTCTGG CCATGGGTTA TAAGCCGAAG GCATATTTGA 1081 GGGATTTTAT GTATGTGTCT CAGGATCCAA AAGATCAACT ATTACTTGGA CCAACATATG 1141 CTACTCCAAA AGTTCTAGAA AAGGCAGGAT TGACCATGAA TGATATTGAT GCTTTTGAAT 1201 TTCATGAAGC TTTCTCGGGT CAGATTTTGG CAAATTTTAA AGCCATGGAT TCTGATTGGT 1261 TTGCAGAAAA CTACATGGGT AGAAAAACCA AGGTTGGATT GCCTCCTTTG GAGAAGTTTA 1321 ATAACTGGGG TGGATCTCTG TCCCTGGGAC ACCCATTTGG AGCCACTGGC TGCAGGTTGG 1381 TCATGGCTGC TGCCAACAGA TTACGGAAAG AAGGAGGCCA GTATGGCTTA GTGGCTGCGT 1441 GTGCAGCTGG AGGGCAGGGC CATGCTATGA TAGTGGAAGC TTATCCAAAA TACCCAACTT 1501 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1561 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1621 CTTGTGGAAA GGACGAAACC GTCGTCTGCT GCTTGTGTTG ACGCGTTAAG TCgacaatca 1681 acctctggat tacaaaattt gtgaaagatt