Transcript: Human NM_000183.3

Homo sapiens hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta (HADHB), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
HADHB (3032)
Length:
1997
CDS:
61..1485

Additional Resources:

NCBI RefSeq record:
NM_000183.3
NBCI Gene record:
HADHB (3032)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000183.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000028040 CGTTAGCCAAACCCAATATAA pLKO.1 194 CDS 100% 15.000 21.000 N HADHB n/a
2 TRCN0000245333 CGTTAGCCAAACCCAATATAA pLKO_005 194 CDS 100% 15.000 21.000 N HADHB n/a
3 TRCN0000245336 TATAAGCCGAAGGCATATTTG pLKO_005 1051 CDS 100% 13.200 18.480 N HADHB n/a
4 TRCN0000028097 GCCAACAGATTACGGAAAGAA pLKO.1 1384 CDS 100% 5.625 4.500 N HADHB n/a
5 TRCN0000245334 CCCTAAGGAAGTAGTTGATTA pLKO_005 339 CDS 100% 13.200 9.240 N HADHB n/a
6 TRCN0000028070 CCTAAGGAAGTAGTTGATTAT pLKO.1 340 CDS 100% 13.200 9.240 N HADHB n/a
7 TRCN0000028110 CGACTGTCTTTAATCTCTAAA pLKO.1 643 CDS 100% 13.200 9.240 N HADHB n/a
8 TRCN0000245335 CGACTGTCTTTAATCTCTAAA pLKO_005 643 CDS 100% 13.200 9.240 N HADHB n/a
9 TRCN0000028067 CGGCTGGAACAGGATGAATAT pLKO.1 772 CDS 100% 13.200 9.240 N HADHB n/a
10 TRCN0000245337 GTTGTCACTAAAGACTAAATG pLKO_005 1741 3UTR 100% 13.200 9.240 N HADHB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000183.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10432 pDONR223 100% 99.7% 99.5% None 3_4insACT n/a
2 ccsbBroad304_10432 pLX_304 0% 99.7% 99.5% V5 3_4insACT n/a
3 ccsbBroadEn_10431 pDONR223 100% 99.6% 99.3% None 3_4insACT;625C>T;825T>C n/a
4 ccsbBroad304_10431 pLX_304 0% 99.6% 99.3% V5 3_4insACT;625C>T;825T>C n/a
5 TRCN0000480893 AACCGTCGTCTGCTGCTTGTGTTG pLX_317 33.7% 99.6% 99.3% V5 3_4insACT;625C>T;825T>C n/a
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