Construct: ORF TRCN0000480908
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005012.1_s317c1
- Derived from:
- ccsbBroadEn_04902
- DNA Barcode:
- CCACGTCTGATCGATGGACCGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OR6B1 (135946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480908
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135946 | OR6B1 | olfactory receptor family 6... | NM_001005281.2 | 100% | 100% | |
| 2 | mouse | 259067 | Olfr449 | olfactory receptor 449 | NM_147064.1 | 86.2% | 92.6% | (many diffs) |
| 3 | mouse | 259067 | Olfr449 | olfactory receptor 449 | XM_011241361.1 | 86.2% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1002
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagttggag aaccagacac gagtcaccaa gttcattctg gtgggattcc 121 ctgggagctt gagtatgcgg gcagccatgt ttctgatatt ccttgtggcc tatattctga 181 cagtggctga aaacgtgatc atcatcctat tggtgctgca aaatcggcca ctgcacaagc 241 ctatgtactt cttcctggcc aacctgtcct tcttggagac ctggtacatc tctgtgactg 301 tgcccaagtt actgtttagt ttttggtctg tgaacaacag catctctttc acactctgta 361 tgatacaact gtacttcttc attgctctca tgtgcacaga atgtgtgctt ctggccgcca 421 tggcctatga ccggtatgtg gccatctgtc gcccactcca ctacccaacc ataatgagcc 481 atgggctctg cttccgcctc gctcttggtt cctgggccat tggctttggc atctccctgg 541 cgaagatcta cttcatctcc tgcctcagct tctgtggtcc caatgtcaTC AACCACTTCT 601 TCTGTGACAT CTCTCCAGTA CTTAATCTCT CCTGCACAGA CATGTCCATA ACTGAGTTGG 661 TAGACTTTAT CCTGGCACTG GTCATCTTCC TATTCCCACT CTTTATTACT GTCCTGTCCT 721 ACGGATGCAT TCTGGCCACC ATATTATGCA TGCCCACAGG AAAGCAGAAA GCGTTCTCCA 781 CTTGTGCCTC CCATCTTGTG GTGGTCACCA TTTTCTATTC AGCCATTATT TTCATGTATG 841 CTCGACCTCG AGTTATCCAT GCCTTCAACA TGAACAAAAT TATTTCCATC TTCTATGCCA 901 TTGTCACTCC TTCTCTCAAC CCTTTCATTT ATTGCCTAAG AAACCGAGAG GTCAAGGAAG 961 CTCTGAAGAA ACTGGCATAT TGCCAGGCCA GCAGATCTGA CTTGCCAACT TTCTTGTACA 1021 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1081 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1141 AGGACGACCA CGTCTGATCG ATGGACCGGA CACGCGTTAA GTCgacaatc aacctctgga 1201 ttacaaaatt tgtgaaagat t