Construct: ORF TRCN0000480919
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019050.3_s317c1
- Derived from:
- ccsbBroadEn_14552
- DNA Barcode:
- GTGGTCTCAGGGCTGCCCGAGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BMX (660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480919
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001721.7 | 100% | 100% | |
| 2 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_203281.3 | 100% | 100% | |
| 3 | human | 660 | BMX | BMX non-receptor tyrosine k... | NM_001320866.2 | 99.8% | 99.8% | 750_751insAGT |
| 4 | human | 660 | BMX | BMX non-receptor tyrosine k... | XM_017029752.2 | 70.6% | 68.2% | 1394_1395ins217;1431_1432ins377 |
| 5 | mouse | 12169 | Bmx | BMX non-receptor tyrosine k... | NM_009759.4 | 85.3% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2094
- ORF length:
- 2025
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatacaaaa tctattctag aagaacttct tctcaaaaga tcacagcaaa 121 agaagaaaat gtcaccaaat aattacaaag aacggctttt tgttttgacc aaaacaaacc 181 tttcctacta tgaatatgac aaaatgaaaa ggggcagcag aaaaggatcc attgaaatta 241 agaaaatcag atgtgtggag aaagtaaatc tcgaggagca gacgcctgta gagagacagt 301 acccatttca gattgtctat aaagatgggc ttctctatgt ctatgcatca aatgaagaga 361 gccgaagtca gtggttgaaa gcattacaaa aagagataag gggtaacccc cacctgctgg 421 tcaagtacca tagtgggttc ttcgtggacg ggaagttcct gtgttgccag cagagctgta 481 aagcagcccc aggatgtacc ctctgggaag catatgctaa tctgcatact gcagtcaatg 541 aagagaaaca cagagttccc accttcccag acagagtgct gaagatacct cgggcagttc 601 ctgttctcaa aatggatgca ccatcttcaa gtaccactct agcccaatat gacaacgaat 661 caaagaaaaa ctatggctcc cagccaccat cttcaagtac cagtctagcg caatatgaca 721 gcaactcaaa gaaaatctat ggctcccagc caaacttcaa catgcagtat attccaaggg 781 aagacttccc tgactggtgg caagtaagaa aactgaaaag tagcagcagc agtgaagatg 841 ttgcaagcag taaccaaaaa gaaagaaatg tgaatcacac cacctcaaag atttcatggg 901 aattccctga gtcaagttca tctgaagaag aggaaaacct ggatgattat gactggtttg 961 ctggtaacat ctccagatca caatctgaac agttactcag acaaaaggga aaagaaggag 1021 catttatggt tagaaattcg agccaagtgg gaatgtacac agtgtcctta tttagtaagg 1081 ctgtgaatga taaaaaagga actgtcaaac attaccacgt gcatacaaat gctgagaaca 1141 aattatacct ggcagaaaac tactgttttg attccattcc aaagcttatt cattatcatc 1201 aacacaattc agcaggcatg atcacacggc tccgccaccc tgtgtcaaca aaggccaaca 1261 aggtccccga ctctgtgtcc ctgggaaatg gaatctggga actgaaaaga gaagagatta 1321 ccttgttgaa ggagctggga agtggccagt ttggagtggt ccagctgggc aagtggaagg 1381 ggcagtatga tgttgctgtt aagatgatca aggagggctc catgtcagaa gatgaattct 1441 ttcaggaggc ccagactatg atgaaactca gccatcccaa gctggttaaa ttctatggag 1501 tgtgttcaaa ggaatacccc atatacatag tgactgaata tataagcaat ggctgcttgc 1561 tgaattacct gaggagtcac ggaaaaggac ttgaaccttc ccagctctta gaaatgtgct 1621 acgatgtctg tgaaggcatg gccttcttgg agagtcacca attcatacac cgggacttGG 1681 CTGCTCGTAA CTGCTTGGTG GACAGAGATC TCTGTGTGAA AGTATCTGAC TTTGGAATGA 1741 CAAGGTATGT TCTTGATGAC CAGTATGTCA GTTCAGTCGG AACAAAGTTT CCAGTCAAGT 1801 GGTCAGCTCC AGAGGTGTTT CATTACTTCA AATACAGCAG CAAGTCAGAC GTATGGGCAT 1861 TTGGGATCCT GATGTGGGAG GTGTTCAGCC TGGGGAAGCA GCCCTATGAC TTGTATGACA 1921 ACTCCCAGGT GGTTCTGAAG GTCTCCCAGG GCCACAGGCT TTACCGGCCC CACCTGGCAT 1981 CGGACACCAT CTACCAGATC ATGTACAGCT GCTGGCACGA GCTTCCAGAA AAGCGTCCCA 2041 CATTTCAGCA ACTCCTGTCT TCCATTGAAC CACTTCGGGA AAAAGACAAG CATCCAACTT 2101 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2161 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2221 CTTGTGGAAA GGACGAGTGG TCTCAGGGCT GCCCGAGAGT ACGCGTTAAG TCgacaatca 2281 acctctggat tacaaaattt gtgaaagatt