Construct: ORF TRCN0000480924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017648.1_s317c1
- Derived from:
- ccsbBroadEn_06153
- DNA Barcode:
- ATAAAAGACTAGCGTCTTGTAACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINB1 (1992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480924
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1992 | SERPINB1 | serpin family B member 1 | NM_030666.4 | 99.9% | 99.7% | 14G>A |
| 2 | human | 1992 | SERPINB1 | serpin family B member 1 | XM_011514333.1 | 99.9% | 99.7% | 14G>A |
| 3 | human | 1992 | SERPINB1 | serpin family B member 1 | XM_011514334.2 | 99.9% | 99.7% | 14G>A |
| 4 | human | 1992 | SERPINB1 | serpin family B member 1 | XM_011514335.2 | 86.7% | 85.4% | (many diffs) |
| 5 | human | 1992 | SERPINB1 | serpin family B member 1 | NR_073112.2 | 45.7% | (many diffs) | |
| 6 | human | 1992 | SERPINB1 | serpin family B member 1 | NR_073111.2 | 43.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcagctgaac tcagcaaaca cccgcttcgc cttggacctg ttcctggcgt 121 tgagtgagaa caatccggct ggaaacatct tcatctctcc cttcagcatt tcatctgcta 181 tggccatggt ttttctgggg accagaggta acacggcagc acagctgtcc aagactttcc 241 atttcaacac ggttgaagag gttcattcaa gattccagag tctgaatgct gatatcaaca 301 aacgtggagc gtcttatatt ctgaaacttg ctaatagatt atatggagag aaaacttaca 361 atttccttcc tgagttcttg gtttcgactc agaaaacata tggtgctgac ctggccagtg 421 tggattttca gcatgcctct gaagatgcaa ggaagaccat aaaccagtgg gtcaaaggac 481 agacagaagg aaaaattccg gaactgttgg cttcgggcat ggttgataac atgaccaaac 541 ttgtgctagt aaatgccatc tatttcaagg gaaactggaa ggataaattc atgaaagaag 601 ccacgacgaa tgcaccattc agattgaata agaaagacag aaaaactgtg aaaatgatgt 661 atcagaagaa aaaatttgca tatggctaca tcgaggacct taagtgccgt gtgctggaac 721 tgccttacca aggcgaggag ctcagcatgg tcatcctgct gccggatgac attgaggacg 781 agtccacggg ccTGAAGAAG ATTGAGGAAC AGTTGACTTT GGAAAAGTTG CATGAGTGGA 841 CTAAACCTGA GAATCTCGAT TTCATTGAAG TTAATGTCAG CTTGCCCAGG TTCAAACTGG 901 AAGAGAGTTA CACTCTCAAC TCCGACCTCG CCCGCCTAGG TGTGCAGGAT CTCTTTAACA 961 GTAGCAAGGC TGATCTGTCT GGCATGTCAG GAGCCAGAGA TATTTTTATA TCAAAAATTG 1021 TCCACAAGTC ATTTGTGGAA GTGAATGAAG AGGGAACAGA GGCGGCAGCT GCCACAGCAG 1081 GCATCGCAAC TTTCTGCATG TTGATGCCCG AAGAAAATTT CACTGCCGAC CATCCATTCC 1141 TTTTCTTTAT TCGGCATAAT TCCTCAGGTA GCATCCTATT CTTGGGGAGA TTTTCTTCCC 1201 CTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAAT AAAAGACTAG CGTCTTGTAA CAACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt