Construct: ORF TRCN0000480943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004959.1_s317c1
- Derived from:
- ccsbBroadEn_11361
- DNA Barcode:
- ATCACACGCGCCGTCGGAGTTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPIG (9360)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9360 | PPIG | peptidylprolyl isomerase G | NM_004792.3 | 97.9% | 97.8% | 240_284del;1335T>A |
| 2 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_005246966.2 | 97.9% | 97.8% | 240_284del;1335T>A |
| 3 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_005246967.1 | 97.9% | 97.8% | 240_284del;1335T>A |
| 4 | human | 9360 | PPIG | peptidylprolyl isomerase G | XM_017005302.2 | 33% | 31.8% | (many diffs) |
| 5 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | NM_001081086.1 | 87.8% | 90.9% | (many diffs) |
| 6 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499184.3 | 87.8% | 90.9% | (many diffs) |
| 7 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499185.3 | 87.8% | 90.9% | (many diffs) |
| 8 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499186.2 | 87.8% | 90.9% | (many diffs) |
| 9 | mouse | 228005 | Ppig | peptidyl-prolyl isomerase G... | XM_006499187.3 | 87.8% | 90.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2286
- ORF length:
- 2217
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggaataaag gttcaacgtc ctcgatgttt ttttgacatt gccattaaca 121 atcaacctgc tggaagagtt gtctttgaat tattttctga tgtgtgcccc aaaacatgcg 181 agaactttcg ttgtctttgt acaggtgaaa aggggaccgg gaaatcaact cagaaaccat 241 tacattataa gagttgtctc tttcacagag ttgtcaagga ttttatggtt caaggtggtg 301 acttcagtga agacgagagt ttcgctgtta aacacaacaa agaatttctc ttgtcaatgg 361 ccaacagagg gaaggataca aatggttcac agttcttcat aacaacgaaa ccaactcctc 421 atttagatgg gcatcatgtt gtttttggac aagtaatctc tggtcaagaa gttgtaagag 481 agattgaaaa ccagaaaaca gatgcagcta gcaaaccgtt tgcggaggta cggatactca 541 gttgtggaga gctgattccc aaatctaaag ttaagaaaga agaaaagaaa aggcataaat 601 catcatcatc ttcctcctcc tcatctagtg actcagatag ctcaagtgat tctcagtcct 661 cttctgattc ctctgattcc gaaagtgcta ctgaagagaa atcaaagaaa agaaaaaaga 721 aacatcggaa aaattcccga aaacacaaga aagaaaagaa aaagcgaaag aaaagcaaga 781 agagtgcatc tagtgagagt gaagctgaaa atcttgaagc acaaccccag tctactgtcc 841 gtccagaaga gatccctcct atacctgaaa atagattcct aatgagaaaa agtcctccta 901 aagctgatga gaaggaaagg aaaaacagag agagagaaag ggaaagagag tgtaatccac 961 ctaactccca gcctgcttca taccagagac gacttttagt tactagatct ggcaggaaaa 1021 ttaaaggaag aggaccaagg cgttatcgaa ctccttccag atccagatca agggatcgtt 1081 tcagacgtag tgagactcct ccacattgga ggcaagagat gcagagagct caaagaatga 1141 gggtatcaag tggtgaaaga tggatcaagg gggataagag tgagttgaat gaaataaaag 1201 aaaatcagag aagtccagtt agagtaaaag agagaaaaat aacagatcac aggaatgtat 1261 ctgagagtcc aaacagaaaa aatgaaaagg agaagaaagt taaagaccat aaatctaaca 1321 gcaaagagag agacatcaga agaaattcag aaaaagaaga caagtataaa aacaaagtga 1381 agaaaagggc caaatctaaa agtaggagta agagcaaaga gaaatcaaag agtaaagaaa 1441 gagattcaaa acataataga aatgaagaaa agaggatgag gtcaaggagt aaaggaaggg 1501 atcatgaaaa tgttaaagaa aaagaaaagc agtctgattc taaaggaaaa gatcaggaaa 1561 ggagtagaag taaagagaag tctaaacagt tagaatcaaa gagtaatgag catgatcaca 1621 gtaaaagtaa ggaaaaggat agacgcgcac aatccaggag tagagaatgt gatataacta 1681 aaggtaaaca cagttataat agcagaacaa gagaacgaag cagaagtagg gacagaagca 1741 gaagagtgcg atcaagaacc catgacagag atcgcagcag aagcaaggag taCCATAGAT 1801 ACAGAGAACA GGAATACAGG AGAAGAGGAC GGTCACGAAG CCGAGAGAGA AGAACACCAC 1861 CAGGAAGATC AAGAAGTAAA GATAGGAGGA GAAGGAGGAG AGACTCACGG AGCTCAGAGA 1921 GAGAAGAAAG TCAAAGCAGA AACAAAGACA AATACAGAAA CCAAGAGAGT AAGAGCTCAC 1981 ACAGAAAAGA AAATTCTGAG AGTGAGAAAA GAATGTACTC TAAAAGTCGT GATCATAATA 2041 GCTCAAATAA CAGCAGGGAA AAAAAGGCTG ATAGAGATCA AAGTCCCTTC TCAAAAATAA 2101 AACAAAGCAG TCAGGACAAT GAATTAAAGT CCTCCATGTT GAAAAATAAG GAGGATGAGA 2161 AGATCAGATC CTCAGTGGAA AAAGAAAACC AAAAATCAAA AGGTCAAGAA AATGACCATG 2221 TACATGAAAA AAATAAAAAA TTTGATCATG AATCAAGCCC TGGAACAGAT GAAGACAAAA 2281 GCGGATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2341 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2401 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATCACACGC GCCGTCGGAG TTCGTACGCG 2461 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt