Construct: ORF TRCN0000480947
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003732.1_s317c1
- Derived from:
- ccsbBroadEn_02112
- DNA Barcode:
- GTATTAGACACCTTGAGACTCTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIAF1 (9220)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480947
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9220 | TIAF1 | TGFB1-induced anti-apoptoti... | NM_004740.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 71
- ORF end:
- 416
- ORF length:
- 345
- Sequence:
-
1 TTTCTTCCAT TTCAGGTGTC GTGAGGCTAG CATCGATTGA TCAACAAGTT TGTACAAAAA 61 AGTTGGCACC ATGTCATCTC CATCTTCACC CTTCAGAGAG CAGTCCTTTC TCTGTGCAGC 121 TGGAGACGCT GGTGAGGAGA GCCGGGTCCA GGTTCTTAAG AATGAGGTGC GGAGGGGCTC 181 TCCGGTGCTG CTGGGCTGGG TTGAGCAAGC CTACGCAGAC AAGTGTGTGT GTGGACCATC 241 CGCACCTCCA GCCCCCACCC CACCCTCTTT GTCTCAGCGT GTTATGTGCA ATGACCTATT 301 TAAGGTAAAC CCATTCCAAC TACAGCAGTT CAGGGCTGAT CCAAGCACTG CCTCCCTCCT 361 GCTCTGTCCA GGTGGTCTGG ACCATAAACT CAACTTGAGA GGGAAGGCTT GGGGTTTGCC 421 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 481 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 541 TATATCTTGT GGAAAGGACG AGTATTAGAC ACCTTGAGAC TCTGAACGCG TTAAGTCgac 601 aatcaacctc tggattacaa aatttgtgaa agatt