Construct: ORF TRCN0000480952

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001539.1_s317c1
Derived from:
ccsbBroadEn_04555
DNA Barcode:
ACACTGTAATCGTGCTCTATTGGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CENPL (91687)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480952
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 91687 CENPL centromere protein L NM_001127181.2 100% 100%
2 human 91687 CENPL centromere protein L NM_001171182.1 88.2% 88.2% 419_420ins138
3 human 91687 CENPL centromere protein L NM_033319.3 88.2% 88.2% 419_420ins138
4 mouse 70454 Cenpl centromere protein L NM_001159930.2 76.1% 74.9% (many diffs)
5 mouse 70454 Cenpl centromere protein L NR_131028.1 38.7% (many diffs)
6 mouse 70454 Cenpl centromere protein L NR_131029.1 35.2% (many diffs)
7 mouse 70454 Cenpl centromere protein L NR_131030.1 32.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1236
ORF length:
1170
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ttcttacagt gcaccagagt caactcctag tgcatcctca agacctgaag
121attactttat aggtgccact cctctgcaga aacgattaga atcggtcagg aagcagagtt
181catttatcct gactccacct cgaaggaaaa ttccccagtg ttcgcagttg caggaagatg
241ttgaccctca aaaggttgca ttccttctgc ataaacagtg gactttatat agtttaactc
301ccttatataa attctcctat agtaatctca aagagtattc tagacttctc aatgctttta
361ttgttgctga aaagcaaaaa ggacttgctg tggaagtggg agaagacttc aacatcaaag
421tgattttttc tactctccta ggaatgaaag gaacacaaag ggacccggaa gcatttcttg
481tccagggtct cattttgtca cccaggctgg agtacagtgg cacgatcttg gttgactgca
541acctctgtct cctgggctca agtgatcctt ccaccttagc cttccaagta gctgggactg
601caggtgcatg ccaccacact cggattgtgt caaaatctca attgccatct gagaatagag
661aaggtaaagt gctgtggact ggctggttct gctgtgtatt tggagacagt cttctggaga
721ctgtttcaga agatttcacc tgtctgccct tattccttgc aaatggagca gagtctaaca
781cagcaataat tggaacttgg tttcagaaaa CCTTTGACTG TTATTTCAGT CCTTTAGCAA
841TCAATGCATT TAATCTTTCC TGGATGGCTG CCATGTGGAC TGCATGCAAA ATGGACCATT
901ATGTGGCTAC TACTGAATTT CTTTGGTCTG TACCCTGTAG CCCTCAAAGT CTGGACATTT
961CTTTCGCAAT ACATCCAGAG GATGCAAAAG CTCTATGGGA CAGTGTCCAC AAAACACCTG
1021GGGAGGTTAC CCAGGAAGAA GTTGACCTAT TCATGGATTG CCTTTATTCA CATTTCCATA
1081GACATTTCAA AATTCATTTA TCAGCCACAA GATTAGTTCG TGTTTCAACA TCTGTAGCTT
1141CAGCACATAC TGATGGAAAA ATAAAGATTC TGTGTCATAA ATACCTTATT GGAGTGTTAG
1201CATATTTGAC AGAACTGGCA ATTTTTCAAA TTGAGTGCCC AACTTTCTTG TACAAAGTGG
1261TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1321CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1381AACACTGTAA TCGTGCTCTA TTGGGACGCG TTAAGTCgac aatcaacctc tggattacaa
1441aatttgtgaa agatt

Download FASTA (ORF) (Full)