Construct: ORF TRCN0000480960
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018819.2_s317c1
- Derived from:
- ccsbBroadEn_14813
- DNA Barcode:
- CTATCGAGCAAAAGCATGCGTCTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKX (5613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480960
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5613 | PRKX | protein kinase X-linked | NM_005044.5 | 99.7% | 31.2% | 128T>C;249C>T;336_337insT |
| 2 | human | 5616 | PRKY | protein kinase Y-linked (ps... | NR_028062.1 | 13.2% | (many diffs) | |
| 3 | human | 441733 | PRKXP1 | PRKX pseudogene 1 | NR_073405.1 | 7.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcgccc gggctggccc aggcggccgc ggcggagagc gactcccgca 121 aggtggcgga ggagaccccc gacggggcgc ccgcgctctg ccccagccct gaggcgctgt 181 cgccggagcc gcctgcgtac agcctgcagg actttgacac gctggccacc gtgggcactg 241 ggacgttcgg gcgggtgcac ctggtgaagg agaagacagc caagcatttc ttcgccctca 301 aggtgatgag cattcctgac gtcatccgcc taaagcagga gcaacacgta cacaatgaga 361 agtctgtcct gaaggaagtc agccacccgt tcctcatcag gctgtttctg gacgtggcat 421 gacgagcgct tcctctacat gctcatggag tacgtgccgg gcggcgagct cttcagctac 481 ctgcgcaacc gggggcgctt ctccagcacc acggggctct tctactctgc agagatcatc 541 tgtgccatcg agtacctgca ctccaaagag atcgtctaca gggacttgaa gccagagaac 601 atcctgctgg atagggatgg ccacattaag ctcacggact ttgggttcgc caagaagctg 661 gtagacagga cttggaccct ctgtggaaca cccgagtacc tagcccccga agtcattcag 721 agcaagggcc acggaagggc cgtggactgg tgggccctcg gcatccTGAT ATTCGAGATG 781 CTTTCGGGGT TTCCTCCGTT TTTTGATGAC AACCCGTTTG GCATTTATCA GAAAATTCTT 841 GCAGGCAAAA TAGATTTCCC CAGACATTTG GATTTCCATG TAAAAGACCT CATTAAGAAA 901 CTGCTCGTGG TTGACAGAAC AAGGCGATTA GGAAACATGA AGAACGGGGC GAATGATGTG 961 AAGCATCATC GGTGGTTCCG CTCCGTGGAC TGGGAAGCTG TTCCGCAGAG AAAACTGAAG 1021 CCTCCCATCG TGCCCAAGAT AGCTGGTGAC GGCGACACTT CCAACTTCGA AACTTACCCT 1081 GAGAATGACT GGGACACAGC CGCGCCCGTG CCGCAGAAGG ATTTAGAAAT CTTCAAGAAT 1141 TTCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC 1201 CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT 1261 TGGCTTTATA TATCTTGTGG AAAGGACGAC TATCGAGCAA AAGCATGCGT CTCACGCGTT 1321 AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att