Construct: ORF TRCN0000480972
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008789.1_s317c1
- Derived from:
- ccsbBroadEn_09337
- DNA Barcode:
- TTAGACCACTTCGGCAAAACTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAF1 (92345)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480972
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | NM_138386.3 | 99.8% | 99.5% | 484A>G;1463C>G |
| 2 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XM_011532410.3 | 99.8% | 99.5% | 484A>G;1463C>G |
| 3 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | NM_001128931.2 | 75.6% | 70.4% | (many diffs) |
| 4 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_938798.3 | 72.4% | (many diffs) | |
| 5 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_938797.3 | 67.7% | (many diffs) | |
| 6 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XM_011532411.3 | 59% | 58.9% | 0_1ins606;857C>G |
| 7 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_938799.3 | 50.5% | (many diffs) | |
| 8 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_938796.3 | 48.2% | (many diffs) | |
| 9 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_001741350.2 | 44.6% | (many diffs) | |
| 10 | human | 92345 | NAF1 | nuclear assembly factor 1 r... | XR_001741349.2 | 42.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1548
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtagtggag gccgccgccg ctcagctgga aactctgaaa ttcaatggca 121 ccgactttgg agttggggaa ggtccggcgg ctccgtctcc gggctctgcc cctgtgccag 181 ggacacagcc gccgctacag tcgtttgagg ggtccccgga cgctgggcag accgtggagg 241 ttaagcctgc cggggagcag cctctgcagc ccgttctgaa cgccgtcgcg gccgggaccc 301 cggcgccgca gccacagcca ccggctgaat cgccggcctg cggagactgc gtcacctccc 361 caggagccgc agagcctgcg cgggcgccgg actccttgga gacctcggac tcggattcgg 421 actcggacag tgaaacagat tcagatagtt caagttcatc gtcttcctct tcatcttcct 481 catcgtcgtc ttcttcctct tgtatatcac ttcctccagt gctgtcagat ggagatgatg 541 atttacaagt tgagaaggaa aataagaatt ttcctcttaa aacaaaagat gaattacttc 601 ttaatgaact gccttctgtt gaagaactca ctattattct gcctgaagat attgagttaa 661 agcctcttgg gatggtttca agtattattg aacaactagt aataattgaa tctatgacta 721 acctacctcc agttaatgag gagactgtaa tttttaaaag tgatcgacaa gcagcaggaa 781 agatattcga gatatttgga cctgttgcac atccatttta tgtgttacgg tttaattctt 841 cagatcacat tgagagtaaa ggtattaaaa taaaggagac tatgtatttt gctccatcaa 901 tgaaagattt cactcaatat atattcacag aaaaactaaa acaggataag ggatcagatg 961 catcatggaa gaatgatcag gaaccaccac cagaggcctt agattttagt gatgatgaaa 1021 aagagaagga agccaaacag aggaaaaaat ctcagattca aggccggaaa aaactcaaat 1081 ctgaatttaa tgagcctggt gaagatttta ctgaagtaca tcagaattgg aatgctcata 1141 gctctgcttc agagcatgca aaaggatatc gtaacagaga attcacacga ggattttcca 1201 gggccagata ccctcgatct tgccatggca ggcctccacc tcagcatttc tataactcag 1261 aacatatggt atctcaggag acttcaggat ttccttctca gagacaaaat aatcccatta 1321 tgccacaata cccctttcct cttccagtgt ttGACATGCA TAATTTTCCC CTCCGCCCTC 1381 CACCCCCACC ACCACCCCCA CCTGTAAACA TGGGTTGGGC TACACCAAAC ATGGCTGCTC 1441 ATCCATTACT TAACTTACCA TACTCCCTAC CCCCACCCCC TCCCCCTCCA CCACTGCCTC 1501 CTCCACCCTC TTCTGGAGAT AGTAATTGTC ATTTTGGACC TTACTATTAC CCAACTTTCT 1561 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1621 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1681 GTGGAAAGGA CGATTAGACC ACTTCGGCAA AACTTTAACG CGTTAAGTCg acaatcaacc 1741 tctggattac aaaatttgtg aaagatt