Construct: ORF TRCN0000480984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002227.1_s317c1
- Derived from:
- ccsbBroadEn_06561
- DNA Barcode:
- CCACTAGCCGGCTGCACGCGCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARS1 (4141)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4141 | MARS1 | methionyl-tRNA synthetase 1 | NM_004990.4 | 99.9% | 99.8% | 1253G>A |
| 2 | human | 4141 | MARS1 | methionyl-tRNA synthetase 1 | XR_001748704.2 | 96.6% | (many diffs) | |
| 3 | human | 4141 | MARS1 | methionyl-tRNA synthetase 1 | XR_002957327.1 | 85.9% | (many diffs) | |
| 4 | human | 4141 | MARS1 | methionyl-tRNA synthetase 1 | XM_006719398.4 | 73.9% | 73.8% | 0_1ins702;551G>A |
| 5 | mouse | 216443 | Mars | methionine-tRNA synthetase | NM_001003913.2 | 87% | 89.3% | (many diffs) |
| 6 | mouse | 216443 | Mars | methionine-tRNA synthetase | NM_001171582.1 | 86.2% | 88.5% | (many diffs) |
| 7 | mouse | 216443 | Mars | methionine-tRNA synthetase | XM_006513568.3 | 55.6% | 56.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2766
- ORF length:
- 2700
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag actgttcgtg agtgatggcg tcccgggttg cttgccggtg ctggccgccg 121 ccgggagagc ccggggcaga gcagaggtgc tcatcagcac tgtaggcccg gaagattgtg 181 tggtcccgtt cctgacccgg cctaaggtcc ctgtcttgca gctggatagc ggcaactacc 241 tcttctccac tagtgcaatc tgccgatatt tttttttgtt atctggctgg gagcaagatg 301 acctcactaa ccagtggctg gaatgggaag cgacagagct gcagccagct ttgtctgctg 361 ccctgtacta tttagtggtc caaggcaaga agggggaaga tgttcttggt tcagtgcgga 421 gagccctgac tcacattgac cacagcttga gtcgtcagaa ctgtcctttc ctggctgggg 481 agacagaatc tctagccgac attgttttgt ggggagccct atacccatta ctgcaagatc 541 ccgcctacct ccctgaggag ctgagtgccc tgcacagctg gttccagaca ctgagtaccc 601 aggaaccatg tcagcgagct gcagagactg tactgaaaca gcaaggtgtc ctggctctcc 661 ggccttacct ccaaaagcag ccccagccca gccccgctga gggaagggct gtcaccaatg 721 agcctgagga ggaggagctg gctaccctat ctgaggagga gattgctatg gctgttactg 781 cttgggagaa gggcctagaa agtttgcccc cgctgcggcc ccagcagaat ccagtgttgc 841 ctgtggctgg agaaaggaat gtgctcatca ccagtgccct cccttacgtc aacaatgtcc 901 cccaccttgg gaacatcatt ggttgtgtgc tcagtgccga tgtctttgcc aggtactctc 961 gcctccgcca gtggaacacc ctctatctgt gtgggacaga tgagtatggt acagcaacag 1021 agaccaaggc tctggaggag ggactaaccc cccaggagat ctgcgacaag taccacatca 1081 tccatgctga catctaccgc tggtttaaca tttcgtttga tatttttggt cgcaccacca 1141 ctccacagca gaccaaaatc acccaggaca ttttccagca gttgctgaaa cgaggttttg 1201 tgctgcaaga tactgtggag caactgcgat gtgagcactg tgctcgcttc ctggctgacc 1261 gcttcgtgga gggcgtgtgt cccttctgtg gctatgagga ggctcggggt gaccagtatg 1321 acaagtgtgg caagctcatc aatgctgtcg agcttaagaa gcctcagtgt aaagtctgcc 1381 gatcatgccc tgtggtgcag tcgagccagc acctgtttct ggacctgcct aagctggaga 1441 agcgactgga ggagtggttg gggaggacat tgcctggcag tgactggaca cccaatgccc 1501 agtttatcac ccgttcttgg cttcgggatg gcctcaagcc acgctgcata acccgagacc 1561 tcaaatgggg aacccctgta cccttagaag gttttgaaga caaggtattc tatgtctggt 1621 ttgatgccac tattggctat ctgtccatca cagccaacta cacagaccag tgggagagat 1681 ggtggaagaa cccagagcaa gtggacctgt atcagttcat ggccaaagac aatgttcctt 1741 tccatagctt agtctttcct tgctcagccc taggagctga ggataactat accttggtca 1801 gccacctcat tgctacagag tacctgaact atgaggatgg gaaattctct aagagccgcg 1861 gtgtgggagt gtttggggac atggcccagg acacggggat ccctgctgac atctggcgct 1921 tctatctgct gtacattcgg cctgagggcc aggacagtgc tttctcctgg acggacctgc 1981 tgctgaagaa taattctgag ctgcttaaca acctgggcaa cttcatcaac agagctggga 2041 tgtttgtgtc taagttcttt gggggctatg tgcctgagat ggtgctcacc cctgatgatc 2101 agcgcctgct ggcccatgtc accctggagc tccagcacta tcaccagcta cttgagaagg 2161 ttcggatccg ggatgccttg cgcagtatcc tcaccatatc tcgacatggc aaccaatata 2221 ttcaggtgaa tgagccctgg aagcggatta aaggcagtga ggctgacagg caacgggcag 2281 gaacagtgac tggcttggca gtgaatatag ctgccttgct ctctgtcatg cttcagcctt 2341 acatgcccac ggttagtgcc acaatccagg cccagctgca gctcccacct ccagccTGCA 2401 GTATCCTGCT GACAAACTTC CTGTGTACCT TACCAGCAGG ACACCAGATT GGCACAGTCA 2461 GTCCCTTGTT CCAAAAATTG GAAAATGACC AGATTGAAAG TTTAAGGCAG CGCTTTGGAG 2521 GGGGCCAGGC AAAAACGTCC CCGAAGCCAG CAGTTGTAGA GACTGTTACA ACAGCCAAGC 2581 CACAGCAGAT ACAAGCGCTG ATGGATGAAG TGACAAAACA AGGAAACATT GTCCGAGAAC 2641 TGAAAGCACA AAAGGCAGAC AAGAACGAGG TTGCTGCGGA GGTGGCGAAA CTCTTGGATC 2701 TAAAGAAACA GTTGGCTGTA GCTGAGGGGA AACCCCCTGA AGCCCCTAAA GGCAAGAAGA 2761 AAAAGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2821 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2881 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCACTAGCC GGCTGCACGC GCTAGACGCG 2941 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt