Transcript: Human XM_006719398.4

PREDICTED: Homo sapiens methionyl-tRNA synthetase 1 (MARS1), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MARS1 (4141)
Length:
2724
CDS:
650..2650

Additional Resources:

NCBI RefSeq record:
XM_006719398.4
NBCI Gene record:
MARS1 (4141)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006719398.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000045662 CTGTACTATTTAGTGGTCCAA pLKO.1 321 5UTR 100% 2.640 3.696 N MARS1 n/a
2 TRCN0000293875 CTAGTGCAATCTGCCGATATT pLKO_005 208 5UTR 100% 0.000 0.000 N MARS1 n/a
3 TRCN0000045659 CCCAGTTTATCACCCGTTCTT pLKO.1 1380 CDS 100% 4.950 3.960 N MARS1 n/a
4 TRCN0000293830 TCGACATGGCAACCAATATAT pLKO_005 2083 CDS 100% 15.000 10.500 N MARS1 n/a
5 TRCN0000045660 CGCTTCTATCTGCTGTACATT pLKO.1 1799 CDS 100% 5.625 3.938 N MARS1 n/a
6 TRCN0000286436 CGCTTCTATCTGCTGTACATT pLKO_005 1799 CDS 100% 5.625 3.938 N MARS1 n/a
7 TRCN0000045661 CAAGGAAACATTGTCCGAGAA pLKO.1 2501 CDS 100% 4.050 2.835 N MARS1 n/a
8 TRCN0000298212 CAAGGAAACATTGTCCGAGAA pLKO_005 2501 CDS 100% 4.050 2.835 N MARS1 n/a
9 TRCN0000045658 CCGCTGGTTTAACATTTCGTT pLKO.1 979 CDS 100% 3.000 2.100 N MARS1 n/a
10 TRCN0000286385 CCGCTGGTTTAACATTTCGTT pLKO_005 979 CDS 100% 3.000 2.100 N MARS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006719398.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06561 pDONR223 100% 73.9% 73.8% None 0_1ins702;551G>A n/a
2 ccsbBroad304_06561 pLX_304 0% 73.9% 73.8% V5 0_1ins702;551G>A n/a
3 TRCN0000480984 CCACTAGCCGGCTGCACGCGCTAG pLX_317 13.9% 73.9% 73.8% V5 0_1ins702;551G>A n/a
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