Construct: ORF TRCN0000480995
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017238.1_s317c1
- Derived from:
- ccsbBroadEn_13722
- DNA Barcode:
- GGACTGCTTCGCGGTTAGATGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS14P3 (644068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480995
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025070.2 | 84.5% | 76.1% | (many diffs) |
| 2 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025071.2 | 84.5% | 76.1% | (many diffs) |
| 3 | human | 6208 | RPS14 | ribosomal protein S14 | NM_005617.4 | 84.5% | 76.1% | (many diffs) |
| 4 | human | 644068 | RPS14P3 | ribosomal protein S14 pseud... | NR_077246.1 | 59.7% | 1_307del;818_853del | |
| 5 | mouse | 102634709 | LOC102634709 | 40S ribosomal protein S14 | XR_001781586.1 | 79.6% | (many diffs) | |
| 6 | mouse | 20044 | Rps14 | ribosomal protein S14 | NM_020600.4 | 78.8% | 76.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acctggaaag gggaaggaaa agaaggaaga acaggtcatc aacctcggac 121 ctcaggtggc tgaaggggag aatgtatttg gtgtctgcca tatctttgca tccttcaatg 181 acacttttgt ccatgtcacg gatctttctg gcaaaCAAAC CATCTGCCGT GTGACTGGTG 241 GGATGAAGGT GAAGGCAGAC CGAGATGAAT CCTCGCCATA TGCTGCTATG TTGACCACCC 301 AGGATGTGGC CCAGAGGTGC AAGGAGCTGG GTATCATTGC CCTACACATC CAACTCCGGG 361 CCACAGGAGG AAATAGGACC AAGACCCTTG GACCTGGGGC CCAGTCGGCC CTCAGAGCCC 421 TTGCCTGCTC GGGTATGAAG ATCGGGAGGA TTGAGGACGT CACCCCCATC CCCTCTGACA 481 GCACTCTCAG GAAGGGGGTC ACCGTGGTCG CCGTCTGTGA ACAAGATTCC TCAAAATATT 541 TTCTGTTAAT AAATTGCCTT CATGTAAAAA ATAAATACCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 AGGACTGCTT CGCGGTTAGA TGTGGACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt