Construct: ORF TRCN0000481001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013897.1_s317c1
- Derived from:
- ccsbBroadEn_03542
- DNA Barcode:
- GCTTTGATCCAGACCCATACCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBCCD1 (55171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481001
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55171 | TBCCD1 | TBCC domain containing 1 | NM_001134415.1 | 100% | 100% | |
| 2 | human | 55171 | TBCCD1 | TBCC domain containing 1 | NM_018138.5 | 100% | 100% | |
| 3 | human | 55171 | TBCCD1 | TBCC domain containing 1 | NM_001286749.2 | 81.8% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1737
- ORF length:
- 1671
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcagtccaga gttctcctct gggtgaaagc agaacccttt atagtgggtg 121 ccttgcaggt cccccctcca tccaagttta gtcttcacta tctcaggaag atatccacct 181 atgtgcaaat ccgggccaca gaaggagctt acccgcgcct ctactggtct acatggaggc 241 acatcgcttg tgggaagctg cagttggcca aggacctggc gtggctttac ttcgaaatat 301 ttgatagtct ttcaatgaag acacctgagg agcgcctgga atggtctgag gttctgtcca 361 actgcatgtc tgaggaggaa gttgaaaagc agagaaatca gctttcagtg gacacgctac 421 agtttctgct cttcttatac attcaacagt tgaacaaggt ctccctaagg acatctttga 481 ttggcgaaga gtggcccagt cccagaaaca aatctcagtc tcctgacctg actgaaaaat 541 ctaattgtca taataagaac tggaatgatt acagtcacca agcttttgtc tatgatcatc 601 tgtctgatct cctcgagctg cttttagatc caaaacaact cactgcatca tttcattcaa 661 cccatagtag tctagtgtct cgagaagctg ttgtggcgct cagcttcctt attgaaggta 721 caataagtag agccaggaag atctatccac ttcatgaact tgcactgtgg caaccactgc 781 atgcagatag tggcttctca aagatctcta agactttctc tttctacaaa ctggaaacct 841 ggttgaggtc ctgtttgact gggaatccat ttggtacatc agcttgcctc aagtctggaa 901 agaaattggc ttgggctcat caagttgaag ggaccaccaa aagagctaag attgcttgta 961 atactcatgt ggcccctagg atgcaccgac tggtagtgat gagccaggtt tacaagcaga 1021 cactggctaa gagctcagac actctggcgg gggcacatgt aaagattcat cgttgcaacg 1081 aatcttttat atatctgctc tctcccttac gatctgtgac aattgagaag tgcaggaata 1141 gcatctttgt cttgggccct gtagggacta cacttcacct ccacagttgt gacaatgtta 1201 aagtcattgc tgtttgccat cgtttgtcca tctcttctac aacaggttgc atctttcacg 1261 ttcttacgcc tacacgccca cttattctct ctgggaacca gacagtaact tttgcccctt 1321 ttcatacaca ttacccaatg ctagaggacc atatggCCAG GACTGGCCTT GCTACAGTGC 1381 CTAACTATTG GGATAATCCA ATGGTTGTGT GCAGAGAGAA CAGCGACACA AGAGTCTTCC 1441 AGCTTTTACC ACCTTGTGAA TTCTATGTAT TTATTATTCC CTTTGAAATG GAAGGGGACA 1501 CAACAGAGAT ACCCGGGGGT CTTCCATCTG TATATCAGAA AGCACTGGGT CAAAGAGAAC 1561 AGAAGATACA GATCTGGCAG AAAACTGTGA AGGAGGCTCA TTTGACAAAG GATCAAAGGA 1621 AGCAGTTCCA GGTACTGGTA GAGAACAAGT TTTATGAATG GTTGATTAAT ACAGGACATC 1681 GCCAACAGCT GGACAGCCTT GTACCCCCTG CAGCAGGCTC CAAACAAGCA GCTGGATACC 1741 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1801 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTGAAAGT ATTTCGATTT CTTGGCTTTA 1861 TATATCTTGT GGAAAGGACG AGCTTTGATC CAGACCCATA CCGTAACGCG TTAAGTCgac 1921 aatcaacctc tggattacaa aatttgtgaa agatt