Construct: ORF TRCN0000481006
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011041.2_s317c1
- Derived from:
- ccsbBroadEn_04813
- DNA Barcode:
- CCTGGTGTGTGGGCTGCCTCATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA11 (126328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481006
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 126328 | NDUFA11 | NADH:ubiquinone oxidoreduct... | NM_175614.5 | 100% | 100% | |
| 2 | human | 126328 | NDUFA11 | NADH:ubiquinone oxidoreduct... | NR_034166.3 | 60.8% | 1_82del;179_298del;626_695del | |
| 3 | human | 126328 | NDUFA11 | NADH:ubiquinone oxidoreduct... | NM_001193375.2 | 53.3% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 492
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgccgaag gtttttcgtc agtactggga tatccccgat ggcaccgatt 121 gccaccgcaa agcctacagc accaccagta ttgccagcgt cgctggcctg accgccgctg 181 ccTACAGAGT CACACTCAAT CCTCCGGGCA CCTTCCTTGA AGGAGTGGCT AAGGTTGGAC 241 AATACACGTT CACTGCAGCT GCTGTCGGGG CCGTGTTTGG CCTCACCACC TGCATCAGCG 301 CCCATGTCCG CGAGAAGCCC GACGACCCCC TGAACTACTT CCTCGGTGGC TGCGCCGGAG 361 GCCTGACTCT GGGAGCACGC ACGCACAACT ACGGGATTGG CGCCGCCGCC TGCGTGTACT 421 TTGGCATAGC GGCCTCCCTG GTCAAGATGG GCCGGCTGGA GGGCTGGGAG GTGTTTGCAA 481 AACCCAAGGT GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT GGTGTGTGGG CTGCCTCATC 661 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t