Construct: ORF TRCN0000481036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014776.1_s317c1
- Derived from:
- ccsbBroadEn_04272
- DNA Barcode:
- CGCGCTGGTGCACCGTAGACTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLT8D2 (83468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481036
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83468 | GLT8D2 | glycosyltransferase 8 domai... | NM_001316967.1 | 100% | 100% | |
| 2 | human | 83468 | GLT8D2 | glycosyltransferase 8 domai... | NM_031302.4 | 100% | 100% | |
| 3 | human | 83468 | GLT8D2 | glycosyltransferase 8 domai... | XM_011538793.3 | 100% | 100% | |
| 4 | human | 83468 | GLT8D2 | glycosyltransferase 8 domai... | XM_017019999.1 | 100% | 100% | |
| 5 | mouse | 74782 | Glt8d2 | glycosyltransferase 8 domai... | NM_029102.3 | 86.7% | 91.7% | (many diffs) |
| 6 | mouse | 74782 | Glt8d2 | glycosyltransferase 8 domai... | XM_006514255.3 | 86.7% | 91.7% | (many diffs) |
| 7 | mouse | 74782 | Glt8d2 | glycosyltransferase 8 domai... | XM_011243591.2 | 86.7% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1113
- ORF length:
- 1047
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctgttacga aaaattaatc aggtgctgct gttccttctg atcgtgaccc 121 tctgtgtgat tctgtataag aaagttcata aggggactgt gcccaagaat gacgcagatg 181 atgaatccga gactcctgaa gaactggaag aagagattcc tgtggtgatt tgtgctgcag 241 cagggaggat gggtgccact atggctgcca tcaatagcat ctacagcaac actgacgcca 301 acatcttgtt ctatgtagtg ggactccgga atactctgac tcgaatacga aaatggattg 361 aacattccaa actgagagaa ataaacttta aaatcgtgga attcaacccg atggtcctca 421 aagggaagat cagaccagac tcatcgaggc ctgaattgct ccagcctctg aactttgttc 481 gattttatct ccctctactt atccaccaac acgagaaagt catctatttg gacgatgatg 541 taattgtaca aggtgatatc caagaactgt atgacaccac cttggccctg ggccacgcgg 601 cggctttctc agatgactgc gatttgccct ctgctcagga cataaacaga ctcgtgggac 661 ttcagaacac atatatgggc tatctggact accggaagaa ggCCATCAAG GACCTTGGCA 721 TCAGCCCCAG CACCTGCTCT TTCAATCCTG GTGTGATTGT TGCCAACATG ACAGAATGGA 781 AGCACCAGCG CATCACCAAG CAATTGGAGA AATGGATGCA AAAGAATGTG GAGGAAAACC 841 TCTATAGCAG CTCCCTGGGA GGAGGGGTGG CCACCTCCCC AATGCTGATT GTGTTTCATG 901 GGAAATATTC CACAATTAAC CCCCTGTGGC ACATAAGGCA CCTGGGCTGG AATCCAGATG 961 CCAGATATTC GGAGCATTTT CTGCAGGAAG CTAAATTACT CCACTGGAAT GGAAGACATA 1021 AACCTTGGGA CTTCCCTAGT GTTCACAACG ACTTATGGGA AAGCTGGTTT GTTCCTGACC 1081 CTGCAGGGAT ATTTAAACTC AATCACCATA GCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1141 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1201 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 1261 CGCTGGTGCA CCGTAGACTG GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1321 ttgtgaaaga tt