Construct: ORF TRCN0000481039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013823.1_s317c1
- Derived from:
- ccsbBroadEn_08115
- DNA Barcode:
- TACATGCCACTTTCTCCCTTTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FHOD1 (29109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481039
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29109 | FHOD1 | formin homology 2 domain co... | NM_013241.3 | 99.9% | 100% | 2478G>T |
| 2 | human | 29109 | FHOD1 | formin homology 2 domain co... | NM_001318202.2 | 97.7% | 97.8% | 1319_1396del;2556G>T |
| 3 | human | 29109 | FHOD1 | formin homology 2 domain co... | XM_011523044.1 | 93.2% | 93.2% | 1319_1396del;2556G>T;2581_2582ins162 |
| 4 | human | 29109 | FHOD1 | formin homology 2 domain co... | XM_011523043.2 | 93.1% | 92.5% | (many diffs) |
| 5 | human | 29109 | FHOD1 | formin homology 2 domain co... | XR_001751895.1 | 90.1% | (many diffs) | |
| 6 | human | 29109 | FHOD1 | formin homology 2 domain co... | XM_011523045.2 | 89.9% | 90% | 0_1ins279;1040_1117del;2277G>T |
| 7 | human | 29109 | FHOD1 | formin homology 2 domain co... | XR_933285.1 | 89.7% | (many diffs) | |
| 8 | human | 29109 | FHOD1 | formin homology 2 domain co... | XR_933286.1 | 78.7% | (many diffs) | |
| 9 | human | 29109 | FHOD1 | formin homology 2 domain co... | XM_006721180.1 | 59.6% | 59.7% | 0_1ins1407;1071G>T |
| 10 | mouse | 234686 | Fhod1 | formin homology 2 domain co... | NM_177699.4 | 82.7% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3558
- ORF length:
- 3492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggcggggaa gaccgcgggg acggagagcc ggtatcagtg gtgaccgtga 121 gggtgcagta cctggaagac accgacccct tcgcatgtgc caactttccg gagccgcgcc 181 gggcccccac ctgcagcctg gacggggcgc tgcccttggg cgcgcagata cccgcggtgc 241 accgcctgct gggagcgccg ctcaagttgg aggattgtgc tctgcaagtg tctccctccg 301 gatactacct ggacaccgag ctgtccctgg aagagcagcg ggagatgctg gagggcttct 361 atgaagagat cagcaaaggg cggaagccca cgctgatcct tcggacccag ctctctgtga 421 gggtcaacgc tatcttggaa aagctgtata gctccagtgg tcctgagctc cgccgctccc 481 tcttctcact gaagcagatc ttccaggagg acaaagacct ggtgcctgaa tttgtgcatt 541 cagaggggct gagctgcctg atccgtgtgg gtgctgctgc cgaccacaac taccagagct 601 acatccttag agcgctcggc cagctgatgc tctttgtgga tggaatgctg ggggtggtgg 661 cccacagtga cactattcag tggctgtaca cattgtgtgc cagcctgtcc cgcttggtgg 721 tgaagacagc cctgaagctg ctgttggtgt ttgtagaata ctccgaaaac aacgcaccgc 781 tgttcatccg tgcagtgaac tctgtggcca gcaccaccgg tgctcctccc tgggccaatc 841 tggtgtccat cctggaggag aagaatggcg ctgaccctga gttgttggtg tacacggtca 901 ccctcatcaa caagacgctg gcggcgctcc cggaccagga ctccttctac gatgtgacgg 961 atgcactgga gcagcagggc atggaagcgc tggtccagcg ccacctgggc actgcgggca 1021 ctgacgtcga cctgcgcacg cagcttgtgc tctacgagaa cgccctgaaa ttggaggatg 1081 gagacatcga agaagcccca ggcgctggtg ggcggcggga acgacgaaag ccttcttctg 1141 aggagggcaa gaggagccgc cgttctctgg aaggcggggg ctgccccgcg cgtgccccgg 1201 aacctggccc cacaggcccc gcctcaccgg taggccccac ctcttccacc ggccccgccc 1261 tgctgacagg ccccgcctcc agccctgtgg gccctccctc cggtctccaa gcttcagtga 1321 acctttttcc taccatctct gtggcaccct cagctgacac ctccagcgag aggagcatct 1381 acaaagcccg gttcctggag aatgtggcgg cagcagaaac agagaagcag gttgcgctgg 1441 cccagggccg ggcagagaca cttgccgggg ccatgcccaa tgaggcgggt ggacacccag 1501 atgcccggca actctgggac tccccagaga cagcccctgc agccagaaca ccccagagcc 1561 ctgccccctg tgtcctgctc cgggcccagc gaagccttgc accagagccc aaggagccac 1621 tgataccagc aagccccaag gctgagccca tctgggagct ccctacccgt gcacccaggc 1681 tctctattgg ggacctggac ttttcagatc taggggagga tgaagaccag gacatgctga 1741 atgtagagtc tgtggaggct gggaaagaca tcccagctcc ctcaccccca ctgcccctgc 1801 tctcgggagt acccccccct cccccacttc cacctccccc acccatcaaa ggccccttcc 1861 caccacctcc acctctacct ctggctgccc ctcttcccca ttcagtgcct gacagctcag 1921 ccctccccac taagaggaag acagtaaaac ttttctggcg tgagctgaag ctggctgggg 1981 gccatggagt ctctgcaagc cgctttgggc cctgcgccac cctctgggct tcactggacc 2041 ctgtctcagt ggacacggcc cgactggaac acctctttga gtctcgtgcc aaagaggtgc 2101 tgccctccaa gaaagctgga gagggccgcc ggacaatgac cacagtgctg gaccccaagc 2161 gcagcaacgc catcaacatc ggcctaacca cactgccacc tgtgcatgtc attaaggctg 2221 ctctgctcaa ctttgatgag tttgctgtca gcaaggatgg cattgagaag ctactgacca 2281 tgatgcccac ggaggaagag cggcagaaga ttgaggaagc ccagctggcc aaccctgaca 2341 tacccctggg cccagccgag aacttcctga tgactcttgc ctccattggc ggcctcgctg 2401 ctcgtctaca actctgggcc ttcaagctgg actatgacag catggagcgg gaaattgctg 2461 agccactgtt tgacctgaaa gtgggtatgg aacagctggt acagaatgcc accttccgct 2521 gcatcctggc taccctccta gctgtgggca acttcctcaa tggctcccag agcagcggct 2581 ttgagctgag ctacctggag aaggtgtcag aggtgaagga cacggtgcgt cgacagtcac 2641 tgctacacca tctctgctcc ctagtgctcc agacccggcc tgagtcctct gacctctatt 2701 cagaaatccc tgccctgacc cgctgtgcca aggtggactt tgaacagctg actgagaacc 2761 tggggcagct ggagcgccgg agccgggcag ccgaggagag cctgcggagc ttggccaagc 2821 atgagctggc cccagccctg cgtgcccgcc tcacccactt cctggaccag tgtgcccgcc 2881 gtgttgccat gctaaggata gtgcaccgcc gtgtctgcaa taggttccat gccttcctgc 2941 tctacctggg ctacaccccg caggcggccc gtgaagtgcg catcatgcag ttctgccaca 3001 cgctgcggga atttgcgctt gagtatcgga cttgccggga acgagtgcta cagcagcagc 3061 agaagcaggc cacataccgt gagcgcaaca agacccgggg acgcatgatc accgagacag 3121 agaagttctc aggtgtggct ggggaagccc ccagcaaccc ctctgtccca gtagcagtga 3181 gcagcgggcc aggccggGGA GATGCTGACA GTCATGCTAG TATGAAGAGT CTGCTGACCA 3241 GCAGGCCTGA GGACACCACA CACAATCGCC GCAGCAGAGG CATGGTCCAG AGCAGCTCCC 3301 CAATCATGCC CACAGTGGGG CCCTCCACTG CATCCCCAGA AGAACCCCCA GGCTCCAGTT 3361 TACCCAGTGA TACATCAGAT GAGATCATGG ACCTTCTGGT GCAGTCAGTG ACCAAGAGCA 3421 GTCCTCGTGC CTTAGCTGCT AGGGAACGCA AGCGTTCCCG CGGCAACCGC AAGTCTTTGA 3481 GAAGGACGTT GAAGAGTGGG CTCGGAGATG ACCTGGTGCA GGCACTGGGA CTAAGCAAGG 3541 GTCCTGGCCT GGAGGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACATGC CACTTTCTCC 3721 CTTTAGGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt