Construct: ORF TRCN0000481040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011927.1_s317c1
- Derived from:
- ccsbBroadEn_01332
- DNA Barcode:
- TAAACACTATCCCGTGTATTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTGER4 (5734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481040
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5734 | PTGER4 | prostaglandin E receptor 4 | NM_000958.3 | 100% | 100% | |
| 2 | human | 5734 | PTGER4 | prostaglandin E receptor 4 | XM_017009658.1 | 64.2% | 57% | (many diffs) |
| 3 | human | 5734 | PTGER4 | prostaglandin E receptor 4 | XM_017009659.2 | 62.5% | 59.3% | (many diffs) |
| 4 | human | 5734 | PTGER4 | prostaglandin E receptor 4 | XM_017009656.2 | 61.5% | 55.7% | 867_958del;1050_1051ins506 |
| 5 | human | 5734 | PTGER4 | prostaglandin E receptor 4 | XM_017009657.1 | 60.4% | 58.2% | 867_924del;978_979ins544 |
| 6 | mouse | 19219 | Ptger4 | prostaglandin E receptor 4 ... | NM_001136079.2 | 85.7% | 87.7% | (many diffs) |
| 7 | mouse | 19219 | Ptger4 | prostaglandin E receptor 4 ... | NM_008965.2 | 81.6% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1533
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccactccc ggggtcaatt cgtccgcctc cttgagcccc gaccggctga 121 acagcccagt gaccatcccg gcggtgatgt tcatcttcgg ggtggtgggc aacctggtgg 181 ccatcgtggt gctgtgcaag tcgcgcaagg agcagaagga gacgaccttc tacacgctgg 241 tatgtgggct ggctgtcacc gacctgttgg gcactttgtt ggtgagcccg gtgaccatcg 301 ccacgtacat gaagggccaa tggcccgggg gccagccgct gtgcgagtac agcaccttca 361 ttctgctctt cttcagcctg tccggcctca gcatcatctg cgccatgagt gtcgagcgct 421 acctggccat caaccatgcc tatttctaca gccactacgt ggacaagcga ttggcgggcc 481 tcacgctctt tgcagtctat gcgtccaacg tgctcttttg cgcgctgccc aacatgggtc 541 tcggtagctc gcggctgcag tacccagaca cctggtgctt catcgactgg accaccaacg 601 tgacggcgca cgccgcctac tcctacatgt acgcgggctt cagctccttc ctcattctcg 661 ccaccgtcct ctgcaacgtg cttgtgtgcg gcgcgctgct ccgcatgcac cgccagttca 721 tgcgccgcac ctcgctgggc accgagcagc accacgcggc cgcggccgcc tcggttgcct 781 cccggggcca ccccgctgcc tccccagcct tgccgcgcct cagcgacttt cggcgccgcc 841 ggagcttccg ccgcatcgcg ggcgccgaga tccagatggt catcttactc attgccacct 901 ccctggtggt gctcatctgc tccatcccgc tcgtggtgcg agtattcgtc aaccagttat 961 atcagccaag tttggagcga gaagtcagta aaaatccaga tttgcaggcc atccgaattg 1021 cttctgtgaa ccccatccta gacccctgga tatatatcct cctgagaaag acagtgctca 1081 gtaaagcaat agagaagatc aaatgcctct tctgccgcat tggcgggtcc cgcagggagc 1141 gctccggaca gcactgcTCA GACAGTCAAA GGACATCTTC TGCCATGTCA GGCCACTCTC 1201 GCTCCTTCAT CTCCCGGGAG CTGAAGGAGA TCAGCAGTAC ATCTCAGACC CTCCTGCCAG 1261 ACCTCTCACT GCCAGACCTC AGTGAAAATG GCCTTGGAGG CAGGAATTTG CTTCCAGGTG 1321 TGCCTGGCAT GGGCCTGGCC CAGGAAGACA CCACCTCACT GAGGACTTTG CGAATATCAG 1381 AGACCTCAGA CTCTTCACAG GGTCAGGACT CAGAGAGTGT CTTACTGGTG GATGAGGCTG 1441 GTGGGAGCGG CAGGGCTGGG CCTGCCCCTA AGGGGAGCTC CCTGCAAGTC ACATTTCCCA 1501 GTGAAACACT GAACTTATCA GAAAAATGTA TATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1681 AACACTATCC CGTGTATTGC CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt