Construct: ORF TRCN0000481043
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008854.2_s317c1
- Derived from:
- ccsbBroadEn_07105
- DNA Barcode:
- ACCCAATAAGCACCCAAAGTAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTC1 (7265)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481043
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7265 | TTC1 | tetratricopeptide repeat do... | NM_001282500.1 | 99.8% | 99.6% | 301G>A |
| 2 | human | 7265 | TTC1 | tetratricopeptide repeat do... | NM_003314.3 | 99.8% | 99.6% | 301G>A |
| 3 | mouse | 66827 | Ttc1 | tetratricopeptide repeat do... | NM_133795.1 | 83.9% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 942
- ORF length:
- 876
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggagaagtca gagaactgtg gggttccaga ggatctgtta aatggtttga 121 aggttacaga tactcaggaa gccgagtgtg ctggccctcc agttcctgat cccaaaaatc 181 agcattccca gagtaagctg ctcagggatg atgaggccca tctccaggag gaccagggag 241 aagaggagtg ttttcatgac tgcagtgcct catttgagga ggagccagga gcggacaagg 301 ttgagaacaa atctaatgaa gatgtgaatt cctctgaact agatgaagaa tacctaatag 361 aactgaaaaa aaacatgtcg gatgaagaga aacagaaaag aagagaagag agcactagac 421 TAAAGGAGGA GGGAAATGAA CAGTTTAAGA AAGGAGATTA TATAGAAGCT GAAAGTTCTT 481 ATAGTCGAGC CCTCGAAATG TGCCCATCCT GCTTCCAAAA GGAGAGGTCG ATTCTATTTT 541 CAAATAGAGC TGCAGCAAGG ATGAAACAGG ACAAGAAAGA AATGGCCATC AATGACTGCA 601 GCAAAGCAAT TCAATTAAAC CCCAGCTATA TCAGGGCAAT ATTGAGGAGA GCAGAGTTGT 661 ATGAGAAGAC GGACAAGCTA GATGAAGCCC TGGAAGACTA TAAATCTATA TTAGAAAAAG 721 ATCCATCAAT ACATCAAGCA AGAGAAGCTT GTATGAGATT ACCTAAGCAA ATTGAAGAAC 781 GTAATGAAAG ACTAAAAGAA GAGATGTTAG GTAAATTAAA AGATCTTGGG AACTTGGTTC 841 TCCGACCTTT TGGGCTCTCC ACGGAAAATT TCCAGATCAA ACAGGATTCC TCTACCGGCT 901 CGTACTCCAT CAATTTCGTT CAAAATCCAA ATAATAACAG ATACTCAACT TTCTTGTACA 961 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1021 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1081 AGGACGAACC CAATAAGCAC CCAAAGTAGC AACGCGTTAA GTCgacaatc aacctctgga 1141 ttacaaaatt tgtgaaagat t