Construct: ORF TRCN0000481045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008529.2_s317c1
- Derived from:
- ccsbBroadEn_10775
- DNA Barcode:
- CATTTCGGAGCTTGAGAGCTTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSDME (1687)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1687 | GSDME | gasdermin E | NM_001127454.2 | 88.8% | 88.8% | 886_996del |
| 2 | human | 1687 | GSDME | gasdermin E | XM_017011802.1 | 88.8% | 88.8% | 886_996del |
| 3 | human | 1687 | GSDME | gasdermin E | NM_001127453.2 | 59.4% | 59.4% | 1_492del;1378_1488del |
| 4 | human | 1687 | GSDME | gasdermin E | NM_004403.3 | 59.4% | 59.4% | 1_492del;1378_1488del |
| 5 | human | 1687 | GSDME | gasdermin E | XM_024446670.1 | 59.4% | 59.4% | 1_492del;1378_1488del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 951
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gaagtgtgtg atctctgagc acatgcaggt cgaggagaag tgtggtggca 121 tcgtgggcat ccagaccaag acggtgcagg tgtcagcgac ggaggatggg aatgtcacca 181 aggactccaa cgtggtgctg gagatcccag ctgccaccac cattgcctac ggtgtcattg 241 agttatacgt gaaactggac ggccagttcg agttctgcct tctccgaggg aagcaaggtg 301 gcttcgagaa caagaagaga attgactctg tctacctgga ccccctggtc tttcgagagt 361 ttgcattcat agacatgcca gatgctgcgc atgggatatc ttcccaggat ggaccattaa 421 gtgttttaaa gcaagcgacc ctgctcctgg agaggaattt ccatccattt gcggagctgc 481 ctgagccaca acagacagct ttgagtgaca tcttccaggc ggtcctattt gatgatgaac 541 tactcatggt cctggaacca gtgtgcgatg acctggTCAG CGGCCTCTCG CCCACAGTGG 601 CGGTGCTGGG GGAGCTGAAG CCCCGGCAGC AGCAGGACCT TGTGGCCTTC CTGCAGCTGG 661 TGGGGTGCAG CTTACAGGGT GGGTGTCCGG GCCCCGAGGA TGCAGGCAGC AAGCAGCTGT 721 TTATGACAGC CTACTTCTTG GTCAGTGCCC TCGCAGAAAT GCCAGATAGC GCAGCAGCTC 781 TGCTGGGCAC TTGCTGCAAA CTCCAGATCA TTCCCACACT GTGCCACTTG CTTCGTGCTC 841 TGTCTGATGA TGGAGTATCT GATCTTGAAG ACCCAACCTT GACTCCCCTG AAAGATACAG 901 AAAGGTTTGG GATTGTGCAG CGCTTGTTTG CCTCAGCTGA CATTAGTCTG TACCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGACATT TCGGAGCTTG AGAGCTTGGT ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt