Construct: ORF TRCN0000481045

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008529.2_s317c1
Derived from:
ccsbBroadEn_10775
DNA Barcode:
CATTTCGGAGCTTGAGAGCTTGGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GSDME (1687)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481045
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1687 GSDME gasdermin E NM_001127454.2 88.8% 88.8% 886_996del
2 human 1687 GSDME gasdermin E XM_017011802.1 88.8% 88.8% 886_996del
3 human 1687 GSDME gasdermin E NM_001127453.2 59.4% 59.4% 1_492del;1378_1488del
4 human 1687 GSDME gasdermin E NM_004403.3 59.4% 59.4% 1_492del;1378_1488del
5 human 1687 GSDME gasdermin E XM_024446670.1 59.4% 59.4% 1_492del;1378_1488del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
951
ORF length:
885
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgca gaagtgtgtg atctctgagc acatgcaggt cgaggagaag tgtggtggca
121tcgtgggcat ccagaccaag acggtgcagg tgtcagcgac ggaggatggg aatgtcacca
181aggactccaa cgtggtgctg gagatcccag ctgccaccac cattgcctac ggtgtcattg
241agttatacgt gaaactggac ggccagttcg agttctgcct tctccgaggg aagcaaggtg
301gcttcgagaa caagaagaga attgactctg tctacctgga ccccctggtc tttcgagagt
361ttgcattcat agacatgcca gatgctgcgc atgggatatc ttcccaggat ggaccattaa
421gtgttttaaa gcaagcgacc ctgctcctgg agaggaattt ccatccattt gcggagctgc
481ctgagccaca acagacagct ttgagtgaca tcttccaggc ggtcctattt gatgatgaac
541tactcatggt cctggaacca gtgtgcgatg acctggTCAG CGGCCTCTCG CCCACAGTGG
601CGGTGCTGGG GGAGCTGAAG CCCCGGCAGC AGCAGGACCT TGTGGCCTTC CTGCAGCTGG
661TGGGGTGCAG CTTACAGGGT GGGTGTCCGG GCCCCGAGGA TGCAGGCAGC AAGCAGCTGT
721TTATGACAGC CTACTTCTTG GTCAGTGCCC TCGCAGAAAT GCCAGATAGC GCAGCAGCTC
781TGCTGGGCAC TTGCTGCAAA CTCCAGATCA TTCCCACACT GTGCCACTTG CTTCGTGCTC
841TGTCTGATGA TGGAGTATCT GATCTTGAAG ACCCAACCTT GACTCCCCTG AAAGATACAG
901AAAGGTTTGG GATTGTGCAG CGCTTGTTTG CCTCAGCTGA CATTAGTCTG TACCCAACTT
961TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1021CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1081CTTGTGGAAA GGACGACATT TCGGAGCTTG AGAGCTTGGT ACGCGTTAAG TCgacaatca
1141acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)