Transcript: Human NM_001127454.2

Homo sapiens gasdermin E (GSDME), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
GSDME (1687)
Length:
2184
CDS:
515..1513

Additional Resources:

NCBI RefSeq record:
NM_001127454.2
NBCI Gene record:
GSDME (1687)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001127454.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083933 GCATGATGAATGACCTGACTT pLKO.1 1644 3UTR 100% 4.950 3.465 N GSDME n/a
2 TRCN0000306831 GCATGATGAATGACCTGACTT pLKO_005 1644 3UTR 100% 4.950 3.465 N GSDME n/a
3 TRCN0000083934 GCGGTCCTATTTGATGATGAA pLKO.1 968 CDS 100% 4.950 3.465 N GSDME n/a
4 TRCN0000083935 GCATTCATAGACATGCCAGAT pLKO.1 812 CDS 100% 4.050 2.835 N GSDME n/a
5 TRCN0000306758 GCATTCATAGACATGCCAGAT pLKO_005 812 CDS 100% 4.050 2.835 N GSDME n/a
6 TRCN0000083937 GATGATGGAGTATCTGATCTT pLKO.1 1295 CDS 100% 4.950 2.970 N GSDME n/a
7 TRCN0000306833 GATGATGGAGTATCTGATCTT pLKO_005 1295 CDS 100% 4.950 2.970 N GSDME n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001127454.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10775 pDONR223 100% 88.8% 88.8% None 886_996del n/a
2 ccsbBroad304_10775 pLX_304 0% 88.8% 88.8% V5 886_996del n/a
3 TRCN0000481045 CATTTCGGAGCTTGAGAGCTTGGT pLX_317 42.5% 88.8% 88.8% V5 886_996del n/a
4 ccsbBroadEn_00440 pDONR223 100% 66.9% 66.9% None 0_1ins492 n/a
5 ccsbBroad304_00440 pLX_304 0% 66.9% 66.9% V5 0_1ins492 n/a
6 TRCN0000472832 ACCTGGGATCTTATTCATTATCTA pLX_317 31.3% 66.9% 66.9% V5 0_1ins492 n/a
7 ccsbBroadEn_06096 pDONR223 100% 66.7% 66.3% None (many diffs) n/a
8 ccsbBroad304_06096 pLX_304 0% 66.7% 66.3% V5 (many diffs) n/a
9 TRCN0000476062 AATTCATTAACCGGCTCGGCACCA pLX_317 24.9% 66.7% 66.3% V5 (many diffs) n/a
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