Construct: ORF TRCN0000481061
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000796.1_s317c1
- Derived from:
- ccsbBroadEn_05185
- DNA Barcode:
- CTTAGTCTTCATCCTCGTCTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM209A (200232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481061
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200232 | FAM209A | family with sequence simila... | NM_001012971.3 | 100% | 100% | |
| 2 | human | 388799 | FAM209B | family with sequence simila... | NM_001013646.4 | 92.9% | 87.7% | (many diffs) |
| 3 | human | 388799 | FAM209B | family with sequence simila... | XM_017027849.1 | 66.5% | 62.7% | (many diffs) |
| 4 | human | 200232 | FAM209A | family with sequence simila... | XR_001754199.1 | 53.9% | 1_45del;559_951del | |
| 5 | human | 388799 | FAM209B | family with sequence simila... | XR_001754273.1 | 20.9% | (many diffs) | |
| 6 | human | 388799 | FAM209B | family with sequence simila... | XR_001754272.1 | 19.2% | (many diffs) | |
| 7 | human | 388799 | FAM209B | family with sequence simila... | XR_001754271.1 | 15.5% | (many diffs) | |
| 8 | human | 388799 | FAM209B | family with sequence simila... | XR_001754270.1 | 12.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 582
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggacgctg aaatcgtccc tggtcctgct tctgtgcctc acctgcagct 121 atgcctttat gttctcttct ctgagacaga aaactagcga accccagggg aaggtgcaat 181 acggagagCA CTTTCGGATT CGGCAGAATC TACCAGAGCA CACCCAAGGC TGGCTTGGGA 241 GCAAATGGCT CTGGCTTCTT TTTGTTGTTG TGCCGTTTGT GATACTGCAG TGTCAAAGAG 301 ACAGTGAGAA GAATAAGGAG CAGAGTCCTC CTGGCCTTCG AGGCGGCCAA CTTCACTCTC 361 CATTAAAGAA AAAAAGAAAT GCTTCCCCCA ACAAAGACTG TGCATTCAAT ACCTTAATGG 421 AACTCGAGGT GGAGCTTATG AAATTTGTGT CCAAAGTGCG GAATCTTAAA CGTGCCATGG 481 CAACAGGTAG TGGCAGTAAC CTCAGGCTTC GAAAGTCAGA GATGCCTGCA GATCCATACC 541 ATGTCACGAT CTGTGAAATA TGGGGAGAAG AAAGCTCTAG CTTGCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGACTT AGTCTTCATC CTCGTCTTAT TACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t