Transcript: Human NM_001013646.4

Homo sapiens family with sequence similarity 209 member B (FAM209B), mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
FAM209B (388799)
Length:
694
CDS:
97..612

Additional Resources:

NCBI RefSeq record:
NM_001013646.4
NBCI Gene record:
FAM209B (388799)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001013646.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000166978 CAATACCTTAAACGAACTTGA pLKO.1 435 CDS 100% 4.950 6.930 N FAM209B n/a
2 TRCN0000172728 GCAGTAACCTCAAGCTTCGAA pLKO.1 521 CDS 100% 3.000 1.800 N FAM209B n/a
3 TRCN0000172679 GCTGTTGTGCCGTTTGTGATA pLKO.1 292 CDS 100% 4.950 2.475 Y FAM209B n/a
4 TRCN0000168414 GTTCTCTTCTCTGAGACAGAA pLKO.1 159 CDS 100% 4.950 2.475 Y FAM209A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001013646.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05185 pDONR223 100% 92.9% 87.7% None (many diffs) n/a
2 ccsbBroad304_05185 pLX_304 0% 92.9% 87.7% V5 (many diffs) n/a
3 TRCN0000481061 CTTAGTCTTCATCCTCGTCTTATT pLX_317 75.3% 92.9% 87.7% V5 (many diffs) n/a
4 ccsbBroadEn_13649 pDONR223 100% 57.5% 46.7% None (many diffs) n/a
5 ccsbBroad304_13649 pLX_304 0% 57.5% 46.7% V5 (many diffs) n/a
6 TRCN0000465475 AAGCAACCACGATGACCGTACCTA pLX_317 100% 57.5% 46.7% V5 (many diffs) n/a
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