Construct: ORF TRCN0000481087
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007170.2_s317c1
- Derived from:
- ccsbBroadEn_07990
- DNA Barcode:
- CCAATTCACCCTGTATCATATCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WIPI2 (26100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481087
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_016003.4 | 99.9% | 100% | 219C>G |
| 2 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_001033519.2 | 97.4% | 97.4% | 219C>G;1164_1165ins33 |
| 3 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_015610.4 | 95.9% | 96% | 75_128del;273C>G |
| 4 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_001033518.2 | 93.5% | 93.6% | 75_128del;273C>G;1218_1219ins33 |
| 5 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_001033520.1 | 85.9% | 83.9% | (many diffs) |
| 6 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | NM_001278299.2 | 71.1% | 71.1% | 0_1ins378 |
| 7 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | XM_006715685.1 | 71.1% | 71.1% | 0_1ins378 |
| 8 | human | 26100 | WIPI2 | WD repeat domain, phosphoin... | XM_017011952.1 | 68.5% | 68.5% | 0_1ins378;786_787ins33 |
| 9 | mouse | 74781 | Wipi2 | WD repeat domain, phosphoin... | NM_178398.4 | 86.2% | 95% | (many diffs) |
| 10 | mouse | 74781 | Wipi2 | WD repeat domain, phosphoin... | XM_006504752.3 | 60.5% | 66.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1374
- ORF length:
- 1308
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cctggcgagc cagagcgggg aggccggcgc cggccagctg ctcttcgcca 121 acttcaacca ggacaacacg tccctagctg ttggtagtaa gtccggttat aaatttttct 181 ccctttcttc tgtggataag ctggaacaga tctatgaatg caccgatacg gaagatgtgt 241 gcattgtaga gagattgttc tccagcagcc tagtggccat cgtgagcctt aaagcaccaa 301 ggaagctaaa ggtttgccac tttaagaagg gaactgagat ctgcaactac agctactcca 361 acacgattct ggctgtgaag ctcaacaggc agaggctgat agtatgcctg gaggagtccc 421 tgtacatcca caacattcgg gacatgaagg tgctgcatac gatcagggag acgcctccaa 481 accctgcagg cctgtgtgcg ctgtcaatca acaacgacaa ctgctacttg gcgtacccag 541 ggagcgcgac catcggagag gtgcaggtct tcgataccat taatttgaga gctgcaaaca 601 tgattccggc tcacgacagt cctttagcgg cactggcctt tgacgcaagt ggaactaaac 661 ttgccacggc ttcggagaag gggaccgtga ttagggtatt ttccattcca gaaggacaaa 721 aactctttga gtttcggaga ggagtaaaga ggtgcgtgag catctgctcc ctggccttca 781 gcatggacgg catgttcctc tccgcctcca gcaacactga gaccgtgcac atcttcaaac 841 tcgagactgt gaaagaaaaa cccccagagg agcccaccac ctggaccggg tacttcggga 901 aagtgctcat ggcctccacc agctacctgc cttcccaagt gacagaaatg ttcaaccagg 961 gcagagccTT CGCCACGGTC CGCCTGCCAT TCTGCGGCCA CAAAAACATC TGCTCGCTAG 1021 CCACAATTCA GAAGATCCCG CGGTTGTTGG TGGGTGCCGC CGACGGGTAC CTGTACATGT 1081 ACAACCTGGA CCCCCAGGAG GGCGGCGAGT GTGCCCTGAT GAAGCAGCAC CGGCTGGACG 1141 GCAGTCTGGA AACGACCAAT GAGATCTTGG ACTCTGCCTC TCACGACTGC CCCTTAGTCA 1201 CTCAGACATA CGGCGCAGCT GCAGGAAAAG GTACTTACGT GCCTTCATCC CCAACGAGAC 1261 TTGCCTACAC AGACGACCTG GGTGCTGTGG GTGGCGCCTG CCTGGAGGAC GAGGCCAGCG 1321 CCCTGCGCCT GGATGAGGAC AGCGAGCACC CGCCCATGAT TCTTCGGACT GACTGCCCAA 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAC CAATTCACCC TGTATCATAT CTTACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att