Transcript: Human NM_001033520.1

Homo sapiens WD repeat domain, phosphoinositide interacting 2 (WIPI2), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
WIPI2 (26100)
Length:
4295
CDS:
233..1387

Additional Resources:

NCBI RefSeq record:
NM_001033520.1
NBCI Gene record:
WIPI2 (26100)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001033520.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153351 GATACGGAAGATGTGTGCATT pLKO.1 269 CDS 100% 4.950 6.930 N WIPI2 n/a
2 TRCN0000153071 GCTGTCAATCAACAACGACAA pLKO.1 544 CDS 100% 4.050 5.670 N WIPI2 n/a
3 TRCN0000154909 GAACTAAACTTGCCACGGCTT pLKO.1 696 CDS 100% 2.160 3.024 N WIPI2 n/a
4 TRCN0000154324 CGGGTACCTGTACATGTACAA pLKO.1 1108 CDS 100% 0.000 0.000 N WIPI2 n/a
5 TRCN0000152278 CCATTAATTTGAGAGCTGCAA pLKO.1 621 CDS 100% 2.640 2.112 N WIPI2 n/a
6 TRCN0000154968 GCCTTCCCAAGTGACAGAAAT pLKO.1 973 CDS 100% 13.200 9.240 N WIPI2 n/a
7 TRCN0000154471 GAGACCGTGCACATCTTCAAA pLKO.1 863 CDS 100% 5.625 3.938 N WIPI2 n/a
8 TRCN0000150787 GCTAAAGGTTTGCCACTTTAA pLKO.1 349 CDS 100% 1.320 0.924 N WIPI2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001033520.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07990 pDONR223 100% 85.9% 83.9% None (many diffs) n/a
2 ccsbBroad304_07990 pLX_304 0% 85.9% 83.9% V5 (many diffs) n/a
3 TRCN0000481087 CCAATTCACCCTGTATCATATCTT pLX_317 31.3% 85.9% 83.9% V5 (many diffs) n/a
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