Construct: ORF TRCN0000481102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016970.1_s317c1
- Derived from:
- ccsbBroadEn_07791
- DNA Barcode:
- AAAAACATCTACCATAAAAAAAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRR4 (11272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481102
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11272 | PRR4 | proline rich 4 | NM_007244.3 | 99.5% | 98.5% | 287G>A;359A>G |
| 2 | human | 11272 | PRR4 | proline rich 4 | NM_001098538.3 | 39.5% | 30.6% | 97_98ins232;127A>G;171_195del |
| 3 | human | 100533464 | PRH1-PRR4 | PRH1-PRR4 readthrough | NR_037918.2 | 23.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 471
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgacaccat gctgctggtc ctgctctcag tggtccttct ggctctgagc TCAGCTCAGA 121 GCACAGATAA TGATGTGAAC TATGAAGACT TTACTTTCAC CATACCAGAT GTAGAGGACT 181 CAAGTCAGAG ACCAGATCAG GGACCCCAGA GACCTCCTCC TGAAGGACTC CTACCTAGAC 241 CCCCTGGTGA TAGTGGTAAC CAAGATGATG GTCCTCAGCA GAGACCACCA AAACCAGGAG 301 GCCATCACCG CCATCCTCCC CCACCTCCTT TTCAAAATCA GCAACGACCA CCCCAACGAG 361 GACACCGTCA ACTCTCTCTA CCCCGATTTC CTTCTGTCAG CCTGCAGGAA GCATCATCAT 421 TCTTCCGGAG GGACAGACCA GCAAGACATC CCCAGGAGCA ACCACTCTGG TTGCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGAAAAA ACATCTACCA TAAAAAAAAC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt