Construct: ORF TRCN0000481109
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010227.1_s317c1
- Derived from:
- ccsbBroadEn_07048
- DNA Barcode:
- CTGGTAAAGACATGTTTGCATAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TF (7018)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481109
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7018 | TF | transferrin | NM_001063.4 | 99.7% | 99.4% | (many diffs) |
| 2 | human | 7018 | TF | transferrin | NM_001354703.2 | 93.4% | 93.1% | (many diffs) |
| 3 | human | 7018 | TF | transferrin | NM_001354704.2 | 81.5% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2160
- ORF length:
- 2094
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gctcgccgtg ggagccctgc tggtctgcgc cgtcctgggg ctgtgtctgg 121 ctgtccctga taaaactgtg agatggtgtg cagtgtcgga gcatgaggcc actaagtgcc 181 agagtttccg cgaccatatg aaaagcgtca ttccatccga tggtcccagt gttgcttgtg 241 tgaagaaagc ctcctacctt gattgcatca gggccattgc ggcaaacgaa gcggatgctg 301 tgacactgga tgcaggtttg gtgtatgatg cttacctggc tcccaataac ctgaagcctg 361 tggtggcaga gttctatggg tcaaaagagg atccacagac tttctattat gctgttgctg 421 tggtgaagaa ggatagtggc ttccagatga accagcttcg aggcaagaag tcctgccaca 481 cgggtctagg caggtccgct gggtggaaca tccccatagg cttactttac tgtgacttac 541 ctgagccacg taaacctctt gagaaagcag tggccaattt cttctcgggc agctgtgccc 601 cttgtgcgga tgggacggac ttcccccagc tgtgtcaact gtgtccaggg tgtggctgct 661 ccacccttaa ccaatacttc ggctactcag gagccttcaa gtgtctgaag aatggtgctg 721 gggatgtggc ctttgtcaag cactcgacta tatttgagaa cttggcaaac aaggctgaca 781 gggaccagta tgagctgctt tgcctggaca acacccggaa gccggtagat gaatacaagg 841 actgccactt ggcccaggtc ccttctcata ccgtcgtggc ccgaagtatg ggcggcaagg 901 aggacttgat ctgggagctt ctcaaccagg cccaggaaca ttttggcaaa gacaaatcaa 961 aagaattcca actattcagc tctcctcatg ggaaggacct gctgtttaag gactctgccc 1021 acgggttttt aaaagtcccc cccaggatgg atgccaagat gtacctgggc tatgagtatg 1081 tcactgccat ccggaatcta cgggaaggca catgccaaga agccccaaca gatgaatgca 1141 agcctgtgaa gtggtgtgcg ctgagccacc acgagaggct caagtgtgat gagtggagtg 1201 ttaacagtgt agggaaaata gagtgtgtat cagcagagac caccgaagac tgcatcgcca 1261 agatcatgaa tggagaagct gatgccatga gcttggatgg agggtttgtc tacatagcgg 1321 gcaagtgtgg tctggtgccn gtcttggcag aaaactacaa taagagcgat aattgtgagg 1381 atacaccaga ggcagggtat tttgctgtag cagtggtgaa gaaatcagct tctgacctca 1441 cctgggacaa tctgaaaggc aagaagtcct gccatacggc agttggcaga accgctggct 1501 ggaacatccc catgggcctg ctctacaata agatcaacca ctgcagattt gatgaatttt 1561 tcagtgaagg ttgtgcccct gggtctaaga aagactccag tctctgtaag ctgtgtatgg 1621 gctcaggcct aaacctgtgt gaacccaaca acaaagaggg atactacggc tacacaggcg 1681 ctttcaggtg tctggttgag aagggagatg tggcctttgt gaaacaccag actgtcccac 1741 agaacactgg gggaaaaaac cctgatccat gggctaagaa tcTGAATGAA AAAGACTATG 1801 AGTTGCTGTG CCTTGATGGT ACCAGGAAAC CTGTGGAGGA GTATGCGAAC TGCCACCTGG 1861 CCAGAGCCCC GAATCACGCT GTGGTCACAC GGAAAGATAA GGAAGCTTGC GTCCACAAGA 1921 TATTACGTCA ACAGCAGCAC CTATTTGGAA GCAACGTAAC TGACTGCTCG GGCAACTTTT 1981 GTTTGTTCCG GTCGGAAACC AAGGACCTTC TGTTCAGAGA TGACACAGTA TGTTTGGCCA 2041 AACTTCATGA CAGAAACACA TATGAAAAAT ACTTAGGAGA AGAATATGTC AAGGCTGTTG 2101 GTAACCTGAG AAAATGCTCC ACCTCATCAC TCCTGGAAGC CTGCACTTTC CGTAGACCTT 2161 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2221 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTGGCT 2281 TTATATATCT TGTGGAAAGG ACGACTGGTA AAGACATGTT TGCATAGCAC GCGTTAAGTC 2341 gacaatcaac ctctggatta caaaatttgt gaaagatt