Construct: ORF TRCN0000481128
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018162.1_s317c1
- Derived from:
- ccsbBroadEn_02086
- DNA Barcode:
- GAGTCATCTCGAAACACTTTATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDLIM1 (9124)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481128
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9124 | PDLIM1 | PDZ and LIM domain 1 | NM_020992.4 | 100% | 100% | |
| 2 | human | 9124 | PDLIM1 | PDZ and LIM domain 1 | XM_011540330.1 | 68.6% | 68.6% | 0_1ins309 |
| 3 | mouse | 54132 | Pdlim1 | PDZ and LIM domain 1 (elfin) | NM_016861.4 | 86.9% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1053
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacccagcag atagacctcc agggcccggg gccgtggggc ttccgcctcg 121 tgggcggcaa ggacttcgag cagcctctcg ccatttcccg ggtcactcct ggaagcaagg 181 cggctctagc taatttatgt attggagatg taatcacagc cattgatggg gaaaatacta 241 gcaatatgac acacttggaa gctcagaaca gaatcaaagg ctgcacagac aacttgactc 301 tcactgtagc cagatctgaa cataaagtct ggtctcctct ggtgacggag gaagggaagc 361 gtcatccata caagatgaat ttagcctctg aaccccagga ggtcctgcac ataggaagcg 421 cccacaaccg aagtgccatg ccctttaccg cctcgcctgc ctccagcact actgccaggg 481 tcatcacaaa ccagtacaac aacccagctg gcctctactc ttctgaaaat atctccaact 541 tcaacaatgc cctggagtca aagactgctg ccagcggggt ggaggcgaac agcagaccct 601 tagaccatgc TCAGCCTCCA AGCAGCCTTG TCATCGACAA AGAATCTGAA GTTTACAAGA 661 TGCTTCAGGA GAAACAGGAG TTGAATGAGC CCCCGAAACA GTCCACGTCT TTCTTGGTTT 721 TGCAGGAAAT CCTGGAGTCT GAAGAAAAAG GGGATCCCAA CAAGCCCTCA GGATTCAGAA 781 GTGTTAAAGC TCCTGTCACT AAAGTGGCTG CGTCGATTGG AAATGCTCAG AAGTTGCCTA 841 TGTGTGACAA ATGTGGCACT GGGATTGTTG GTGTGTTTGT GAAGCTGCGG GACCGTCACC 901 GCCACCCTGA GTGTTATGTG TGCACTGACT GTGGCACCAA CCTGAAACAG AAGGGCCATT 961 TCTTTGTGGA GGATCAAATC TACTGTGAGA AGCATGCCCG GGAGCGAGTC ACACCACCTG 1021 AGGGTTATGA AGTGGTCACT GTGTTCCCCA AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1081 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1141 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 1201 GTCATCTCGA AACACTTTAT CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1261 ttgtgaaaga tt