Construct: ORF TRCN0000481134
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016006.1_s317c1
- Derived from:
- ccsbBroadEn_15485
- DNA Barcode:
- AGGAGTCATAAACATACAACGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IREB2 (3658)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481134
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3658 | IREB2 | iron responsive element bin... | NM_001320943.2 | 99.9% | 100% | 999A>T |
| 2 | human | 3658 | IREB2 | iron responsive element bin... | NM_004136.4 | 35.5% | 35.4% | (many diffs) |
| 3 | human | 3658 | IREB2 | iron responsive element bin... | NM_001320942.2 | 29.6% | 29.4% | (many diffs) |
| 4 | human | 3658 | IREB2 | iron responsive element bin... | NM_001354994.1 | 29.6% | 29.4% | (many diffs) |
| 5 | mouse | 64602 | Ireb2 | iron responsive element bin... | XM_006511335.3 | 43.5% | 43.9% | (many diffs) |
| 6 | mouse | 64602 | Ireb2 | iron responsive element bin... | XM_017313500.1 | 40.8% | 41.2% | (many diffs) |
| 7 | mouse | 64602 | Ireb2 | iron responsive element bin... | NM_022655.3 | 32.9% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccccaaaa gcaggatacg cctttgagta ccttattgaa acattaaatg 121 acagttcaca taagaagttc ttcgatgtat ctaaacttgg caccaagtat gatgttctgc 181 cttactcaat acgggtcttg ttggaagctg ctgtacgaaa ttgtgatggc tttttaatga 241 agaaggaaga tgttatgaac attttagact ggaaaaccaa acaaagcaat gttgaagtgc 301 cctttttccc tgcccgtgtt cttcttcaag attttactgg aataccagca atggtggatt 361 ttgctgctat gagggaggca gtgaaaactc ttggaggtga tcctgagaaa gtccatcctg 421 cttgtccgac agatcttaca gttgaccatt ctttacaaat tgacttcagt aaatgtgcaa 481 tacagaatgc accaaatcct ggaggtggtg acctgcagaa agcaggaaag ctctctccac 541 ttaaagtgca gcctaagaag cttccctgca gaggccagac tacctgccga ggatcttgtg 601 attctggaga actaggccga aactcaggaa cattttcttc gcagattgag aatacaccca 661 tcctgtgtcc ttttcatttg caaccagtgc ctgaaccTGA AACAGTGTTA AAAAATCAAG 721 AAGTAGAATT CGGCAGAAAT CGAGAGAGGC TTCAGTTTTT TAAGTGGAGT TCAAGAGTTT 781 TTAAGAATGT GGCAGTGATC CCTCCTGGAA CTGGAATGGC TCATCAAATA AACTTAGAAT 841 ATTTGTCAAG AGTGGTTTTT GAAGAAAAAG ACCTCCTCTT CCCAGACAGT GTAGTCGGCA 901 CAGATTCACA CATAACGATG GTGAATGGTT TAGGGATTCT GGGGTGGGGG GTTGGAGGCA 961 TTGAAACAGA AGCAGTTATG CTTGGTCTGC CAGTTTCTCT TACTTTACCA GAGGTGGTTG 1021 GATGTGAGTT AACTGGGTCA TCAAACCCTT TTGTTACATC CATTGATGTT GTTCTTGGTA 1081 TTACAAAGGT AAGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1141 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1201 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AGGAGTCATA AACATACAAC 1261 GTTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt