Construct: ORF TRCN0000481139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014733.1_s317c1
- Derived from:
- ccsbBroadEn_12246
- DNA Barcode:
- GGTCTAAAGTTATAACAAACTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP4R3A (55671)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481139
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001284281.1 | 100% | 100% | |
| 2 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001366432.1 | 71.3% | 71.3% | 196_912del |
| 3 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XM_005267842.2 | 71.3% | 71.3% | 196_912del |
| 4 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NM_001284280.1 | 69.7% | 69.7% | 196_912del;1227_1228ins39 |
| 5 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_943491.3 | 39.9% | (many diffs) | |
| 6 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_002957561.1 | 39.6% | (many diffs) | |
| 7 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_001750421.2 | 39% | (many diffs) | |
| 8 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | XR_002957560.1 | 39% | (many diffs) | |
| 9 | human | 55671 | PPP4R3A | protein phosphatase 4 regul... | NR_158976.1 | 38.9% | (many diffs) | |
| 10 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | NM_001160214.1 | 66.7% | 70.7% | (many diffs) |
| 11 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XM_017315173.1 | 66% | 69.8% | (many diffs) |
| 12 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | NM_211355.2 | 65.2% | 69.1% | (many diffs) |
| 13 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XM_017315174.1 | 64.5% | 68.2% | (many diffs) |
| 14 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XR_001780474.1 | 52% | (many diffs) | |
| 15 | mouse | 68734 | Ppp4r3a | protein phosphatase 4 regul... | XR_381543.3 | 47.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1848
- ORF length:
- 1782
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgacacccgg cggcgggtga aggtgtacac gctcaacgag gaccggcagt 121 gggacgaccg gggcaccggg catgtgtcgt ctggctacgt ggagcggctg aagggcatgt 181 ccctgcttgt cagggctgag agcgacggtt ctctactttt agagtcgaaa ataaatccta 241 acactgcata ccagaaacaa caggaagatg aaaaatttct gacagatttg tttgcacaac 301 taacagatga agcaacagat gaggaaaaaa gacaggaatt ggttaacttt ttaaaagaat 361 tttgtgcgtt ttcccaaacg ctacagcctc aaaacagaga tgcttttttc aagactttgt 421 caaacatggg catattacca gctttagaag tcatccttgg catggatgat acacaggtgc 481 gaagtgctgc tactgatata ttctcatact tggttgaata taatccatcc atggtacgag 541 agtttgtcat gcaggaggca caacagaatg atgatgtaag taagaagtta acagagcaaa 601 aaataaccag caaggatatt ttgctcatca acctcattat agaacatatg atttgtgata 661 cagatcctga acttggagga gcagtccagc ttatgggcct gcttcgaact ttagttgacc 721 cagagaacat gctagccact gccaataaaa cagaaaagac tgaatttctg ggtttcttct 781 acaagcactg tatgcatgtt ctcactgctc ctttactagc aaatacaaca gaagacaaac 841 ctagtaaaga tgattttcag actgcccaac tattggcact tgtattggaa ttgttaacat 901 tttgtgtgga gcaccatacc taccacataa agaactacat tattaataag gatatcctcc 961 ggagagtgct agttcttatg gcctcgaagc atgctttctt ggcattatgt gcccttcgtt 1021 ttaaaagaaa gattattgga ttaaaagatg agttttacaa ccgctacata atgaaaagtt 1081 ttttgtttga accagtagtg aaagcatttc tcaacaatgg atcccgctac aatctgatga 1141 actctgccat aatagagatg tttgaattta ttagagtgga agatataaaa tcattaactg 1201 ctcatgtaat tgaaaattac tggaaagcac tggaagatgt agattatgta cagacattta 1261 aaggattaaa actgagattt gaacaacaaa gagaaaggca agataatccc aaacttgaca 1321 gtatgcgttc cattttgagg aatcacagat atcgaagaga tgccagaaca ctagaagatg 1381 aagaagagat gtggtttaac acagatgaag atgacatgga agatggagaa gctgtagtgt 1441 ctccatctga caaaactaaa aatgatgatg atattatgga tcCAATAAGT AAATTCATGG 1501 AAAGGAAGAA ATTAAAAGAA AGTGAGGAAA AGGAAGTGCT TCTGAAAACA AACCTTTCTG 1561 GACGGCAGAG CCCAAGTTTC AAGCTTTCCC TGTCCAGTGG AACGAAGACT AACCTCACCA 1621 GCCAGTCATC TACAACAAAT CTGCCTGGTT CTCCGGGATC ACCTGGATCC CCAGGATCTC 1681 CAGGCTCTCC TGGATCCGTA CCTAAAAATA CATCTCAGAC GGCAGCTATT ACTACAAAGG 1741 GAGGCCTCGT GGGTCTGGTA GATTATCCTG ATGATGATGA AGATGATGAT GAGGATGAAG 1801 ATAAGGAAGA TACGTTACCA TTGTCAAAGA AAGCAAAATT TGATTCATAC CCAACTTTCT 1861 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1921 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1981 GTGGAAAGGA CGAGGTCTAA AGTTATAACA AACTGGTACG CGTTAAGTCg acaatcaacc 2041 tctggattac aaaatttgtg aaagatt