Construct: ORF TRCN0000481145
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005581.1_s317c1
- Derived from:
- ccsbBroadEn_08049
- DNA Barcode:
- GGGAGCCGCTTTGCGACGCCCTTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- STAU2 (27067)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481145
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164381.1 | 99.9% | 496G>A | |
| 2 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164380.2 | 93.4% | (many diffs) | |
| 3 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164384.1 | 88.9% | 496G>A;1436T>C;1437_1438ins177 | |
| 4 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_014393.2 | 88.9% | 496G>A;1436T>C;1437_1438ins177 | |
| 5 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164385.1 | 87.7% | (many diffs) | |
| 6 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164382.1 | 80.8% | 0_1ins216;280G>A;1215_1328del | |
| 7 | human | 27067 | STAU2 | staufen double-stranded RNA... | NM_001164383.1 | 73.9% | 0_1ins420;76G>A | |
| 8 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001111272.1 | 91% | (many diffs) | |
| 9 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495532.3 | 91% | (many diffs) | |
| 10 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238389.1 | 91% | (many diffs) | |
| 11 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_025303.3 | 85.7% | (many diffs) | |
| 12 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_006495528.3 | 85.7% | (many diffs) | |
| 13 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238387.1 | 85.7% | (many diffs) | |
| 14 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321102.1 | 85.7% | (many diffs) | |
| 15 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_017321109.1 | 82.9% | (many diffs) | |
| 16 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347045.1 | 81.2% | (many diffs) | |
| 17 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238388.2 | 79.2% | (many diffs) | |
| 18 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | XM_011238390.2 | 78.1% | (many diffs) | |
| 19 | mouse | 29819 | Stau2 | staufen (RNA binding protei... | NM_001347044.1 | 76.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 58
- ORF end:
- 76
- ORF length:
- 18
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg 61 ttggcatgct tcaaataaat cagatgttct cagtgcagct gagtcttggt gagcagacat 121 gggaatccga aggcagcagt ataaagaagg ctcagcaggc tgttgccaat aaagctttga 181 ctgaatctac gcttcccaaa ccagttcaga agccacccaa aagtaatgtt aacaataacc 241 caggcagtat aactccaact gtggaactga atgggcttgc tatgaaaagg ggagagcctg 301 ccatctacag gccattagat ccaaagccat tcccaaatta tagagctaat tacaactttc 361 ggggcatgta caatcagagg tatcattgcc cagtgcctaa gatcttttat gttcagctca 421 ctgtaggaaa taatgaattt tttggggaag gaaagactcg acaagctgct agacacaatg 481 ctgcaatgaa agccctccaa gcactgcaga atgaacctat tccagaaaga tctcctcaga 541 atggtgaatc aggaaaggat atggatgatg acaaagatgc aaataagtct gagatcagct 601 tagtgtttga aattgctctg aagcgaaata tgcctgtcag ttttgaggtt attaaagaaa 661 gtggaccacc acatatgaaa agctttgtta ctcgagtgtc agtaggagag ttctctgcag 721 aaggagaagg aaatagcaaa aaactctcca agaagcgcgc tgcgaccacc gtcttacagg 781 agcttaaaaa acttccacct cttcctgtgg tggaaaagcc aaaactattt tttaaaaaac 841 gccctaaaac aatagtaaag gccggaccag aatatggcca agggatgaac cctattagcc 901 gcctggcgca aattcaacag gccaaaaagg aaaaggagcc ggattatgtt ttgctttcag 961 aaagaggaat gcctcgacgt cgagaatttg tgatgcaggt gaaggtaggc aatgaagttg 1021 ctacaggaac aggacctaat aaaaagatag ccaaaaaaaa tgctgcagaa gcaatgctgt 1081 tacaacttgg ttataaagca tccactaatc ttcaggatca acttgagaag acaggggaaa 1141 acaaaggatg gagtggtcca aagcctgggt ttcctgaacc aacaaataat actccaaaag 1201 gaattcttca tttgtctcct gatgtttatc aagagatgga agccagccgc cacaaagtaa 1261 tcTCTGGCAC TACTCTAGGC TATTTGTCAC CCAAAGATAT GAACCAACCT TCAAGCTCTT 1321 TCTTCAGTAT ATCTCCCACA TCGAATAGTT CAGCTACAAT TGCCAGGGAA CTCCTTATGA 1381 ATGGAACATC TTCTACAGCT GAAGCCATAG GTTTAAAAGG AAGTTCTCCT ACTCCCCCTT 1441 GTTCTCCAGT ACAACCTTCA AAACAACTGG AATATTTAGC AAGGATTCAA GGCTTTCAGG 1501 CAGCCTTAAG TGCCTTGAAA CAATTTTCTG AACAAGGACT GGATCCAATC GATGGAGCAA 1561 TGAATATCGA AAAAGGTTCT CTTGAAAAAC AAGCCAAGCA TCTGAGAGAG AAAGCGGACA 1621 ATAACCAGGC ACCCCCGGGC TCCATCGCTC AGGACTGCAA GAAATCAAAC TCGGCCGTCT 1681 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1741 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1801 TTTATATATC TTGTGGAAAG GACGAGGGAG CCGCTTTGCG ACGCCCTTGA CGCGTTAAGT 1861 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt