Construct: ORF TRCN0000481145

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005581.1_s317c1
Derived from:
ccsbBroadEn_08049
DNA Barcode:
GGGAGCCGCTTTGCGACGCCCTTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
STAU2 (27067)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481145
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27067 STAU2 staufen double-stranded RNA... NM_001164381.1 99.9% 496G>A
2 human 27067 STAU2 staufen double-stranded RNA... NM_001164380.2 93.4% (many diffs)
3 human 27067 STAU2 staufen double-stranded RNA... NM_001164384.1 88.9% 496G>A;1436T>C;1437_1438ins177
4 human 27067 STAU2 staufen double-stranded RNA... NM_014393.2 88.9% 496G>A;1436T>C;1437_1438ins177
5 human 27067 STAU2 staufen double-stranded RNA... NM_001164385.1 87.7% (many diffs)
6 human 27067 STAU2 staufen double-stranded RNA... NM_001164382.1 80.8% 0_1ins216;280G>A;1215_1328del
7 human 27067 STAU2 staufen double-stranded RNA... NM_001164383.1 73.9% 0_1ins420;76G>A
8 mouse 29819 Stau2 staufen (RNA binding protei... NM_001111272.1 91% (many diffs)
9 mouse 29819 Stau2 staufen (RNA binding protei... XM_006495532.3 91% (many diffs)
10 mouse 29819 Stau2 staufen (RNA binding protei... XM_011238389.1 91% (many diffs)
11 mouse 29819 Stau2 staufen (RNA binding protei... NM_025303.3 85.7% (many diffs)
12 mouse 29819 Stau2 staufen (RNA binding protei... XM_006495528.3 85.7% (many diffs)
13 mouse 29819 Stau2 staufen (RNA binding protei... XM_011238387.1 85.7% (many diffs)
14 mouse 29819 Stau2 staufen (RNA binding protei... XM_017321102.1 85.7% (many diffs)
15 mouse 29819 Stau2 staufen (RNA binding protei... XM_017321109.1 82.9% (many diffs)
16 mouse 29819 Stau2 staufen (RNA binding protei... NM_001347045.1 81.2% (many diffs)
17 mouse 29819 Stau2 staufen (RNA binding protei... XM_011238388.2 79.2% (many diffs)
18 mouse 29819 Stau2 staufen (RNA binding protei... XM_011238390.2 78.1% (many diffs)
19 mouse 29819 Stau2 staufen (RNA binding protei... NM_001347044.1 76.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
58
ORF end:
76
ORF length:
18
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaatg
61ttggcatgct tcaaataaat cagatgttct cagtgcagct gagtcttggt gagcagacat
121gggaatccga aggcagcagt ataaagaagg ctcagcaggc tgttgccaat aaagctttga
181ctgaatctac gcttcccaaa ccagttcaga agccacccaa aagtaatgtt aacaataacc
241caggcagtat aactccaact gtggaactga atgggcttgc tatgaaaagg ggagagcctg
301ccatctacag gccattagat ccaaagccat tcccaaatta tagagctaat tacaactttc
361ggggcatgta caatcagagg tatcattgcc cagtgcctaa gatcttttat gttcagctca
421ctgtaggaaa taatgaattt tttggggaag gaaagactcg acaagctgct agacacaatg
481ctgcaatgaa agccctccaa gcactgcaga atgaacctat tccagaaaga tctcctcaga
541atggtgaatc aggaaaggat atggatgatg acaaagatgc aaataagtct gagatcagct
601tagtgtttga aattgctctg aagcgaaata tgcctgtcag ttttgaggtt attaaagaaa
661gtggaccacc acatatgaaa agctttgtta ctcgagtgtc agtaggagag ttctctgcag
721aaggagaagg aaatagcaaa aaactctcca agaagcgcgc tgcgaccacc gtcttacagg
781agcttaaaaa acttccacct cttcctgtgg tggaaaagcc aaaactattt tttaaaaaac
841gccctaaaac aatagtaaag gccggaccag aatatggcca agggatgaac cctattagcc
901gcctggcgca aattcaacag gccaaaaagg aaaaggagcc ggattatgtt ttgctttcag
961aaagaggaat gcctcgacgt cgagaatttg tgatgcaggt gaaggtaggc aatgaagttg
1021ctacaggaac aggacctaat aaaaagatag ccaaaaaaaa tgctgcagaa gcaatgctgt
1081tacaacttgg ttataaagca tccactaatc ttcaggatca acttgagaag acaggggaaa
1141acaaaggatg gagtggtcca aagcctgggt ttcctgaacc aacaaataat actccaaaag
1201gaattcttca tttgtctcct gatgtttatc aagagatgga agccagccgc cacaaagtaa
1261tcTCTGGCAC TACTCTAGGC TATTTGTCAC CCAAAGATAT GAACCAACCT TCAAGCTCTT
1321TCTTCAGTAT ATCTCCCACA TCGAATAGTT CAGCTACAAT TGCCAGGGAA CTCCTTATGA
1381ATGGAACATC TTCTACAGCT GAAGCCATAG GTTTAAAAGG AAGTTCTCCT ACTCCCCCTT
1441GTTCTCCAGT ACAACCTTCA AAACAACTGG AATATTTAGC AAGGATTCAA GGCTTTCAGG
1501CAGCCTTAAG TGCCTTGAAA CAATTTTCTG AACAAGGACT GGATCCAATC GATGGAGCAA
1561TGAATATCGA AAAAGGTTCT CTTGAAAAAC AAGCCAAGCA TCTGAGAGAG AAAGCGGACA
1621ATAACCAGGC ACCCCCGGGC TCCATCGCTC AGGACTGCAA GAAATCAAAC TCGGCCGTCT
1681ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1741GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1801TTTATATATC TTGTGGAAAG GACGAGGGAG CCGCTTTGCG ACGCCCTTGA CGCGTTAAGT
1861Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)