Construct: ORF TRCN0000481177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005667.1_s317c1
- Derived from:
- ccsbBroadEn_01625
- DNA Barcode:
- CCTCAATGGGATATCTACTCTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SURF4 (6836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6836 | SURF4 | surfeit 4 | NM_033161.4 | 100% | 100% | |
| 2 | human | 6836 | SURF4 | surfeit 4 | XM_011518943.1 | 96.2% | 94.4% | (many diffs) |
| 3 | human | 6836 | SURF4 | surfeit 4 | XM_011518944.2 | 96.2% | 94.4% | (many diffs) |
| 4 | human | 6836 | SURF4 | surfeit 4 | NM_001280788.1 | 94.1% | 94% | 0_1ins46;2delT |
| 5 | human | 6836 | SURF4 | surfeit 4 | NM_001280790.1 | 84% | 84% | 0_1ins129 |
| 6 | human | 6836 | SURF4 | surfeit 4 | NM_001280791.1 | 84% | 84% | 0_1ins129 |
| 7 | human | 6836 | SURF4 | surfeit 4 | XM_011518945.3 | 73.6% | 32.3% | (many diffs) |
| 8 | human | 6836 | SURF4 | surfeit 4 | NM_001280789.2 | 58.9% | 58.7% | (many diffs) |
| 9 | human | 6836 | SURF4 | surfeit 4 | NM_001280793.1 | 56.5% | 56.5% | 0_1ins351 |
| 10 | human | 6836 | SURF4 | surfeit 4 | NM_001280792.1 | 47.5% | 44.6% | 356_357ins187;384_385ins236 |
| 11 | human | 6836 | SURF4 | surfeit 4 | XM_017015062.1 | 40.7% | 34% | (many diffs) |
| 12 | mouse | 20932 | Surf4 | surfeit gene 4 | NM_011512.3 | 90.2% | 99.2% | (many diffs) |
| 13 | mouse | 20932 | Surf4 | surfeit gene 4 | XM_006497828.3 | 75.3% | 83.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccagaacgac ctgatgggca cggccgagga cttcgccgac cagttcctcc 121 gtgtcacaaa gcagtacctg ccccacgtgg cgcgcctctg tctgatcagc accttcctgg 181 aggacggcat ccgtatgtgg ttccagtgga gcgagcagcg cgactacatc gacaccacct 241 ggaactgcgg ctacctgctg gcctcgtcct tcgtcttcct caacttgctg ggacagctga 301 ctggctgcgt cctggtgttg agcaggaact tcgtgcagta cgcctgcttc gggctctttg 361 gaatcatagc tctgcagacg attgcctaca gcattttatg ggacttgaag tttttgatga 421 ggaacctggc cctgggagga ggCCTGTTGC TGCTCCTAGC AGAATCCCGT TCTGAAGGGA 481 AGAGCATGTT TGCGGGCGTC CCCACCATGC GTGAGAGCTC CCCCAAACAG TACATGCAGC 541 TCGGAGGCAG GGTCTTGCTG GTTCTGATGT TCATGACCCT CCTTCACTTT GACGCCAGCT 601 TCTTTTCTAT TGTCCAGAAC ATCGTGGGCA CAGCTCTGAT GATTTTAGTG GCCATTGGTT 661 TTAAAACCAA GCTGGCTGCT TTGACTCTTG TTGTGTGGCT CTTTGCCATC AACGTATATT 721 TCAACGCCTT CTGGACCATT CCAGTCTACA AGCCCATGCA TGACTTCCTG AAATACGACT 781 TCTTCCAGAC CATGTCGGTG ATTGGGGGCT TGCTCCTGGT GGTGGCCCTG GGCCCTGGGG 841 GTGTCTCCAT GGATGAGAAG AAGAAGGAGT GGTACCCAAC TTTCTTGTAC AAAGTGGTTG 901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1021 TCAATGGGAT ATCTACTCTC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 1081 ttgtgaaaga tt