Construct: ORF TRCN0000481191
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014341.1_s317c1
- Derived from:
- ccsbBroadEn_12876
- DNA Barcode:
- TTGCGCTGCGACAGTTGATCTCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLXDC2 (84898)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481191
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84898 | PLXDC2 | plexin domain containing 2 | NM_001282736.2 | 100% | 100% | |
| 2 | human | 84898 | PLXDC2 | plexin domain containing 2 | NM_032812.9 | 90.7% | 90.7% | 325_471del |
| 3 | human | 84898 | PLXDC2 | plexin domain containing 2 | XM_011519750.2 | 81.8% | 76.9% | (many diffs) |
| 4 | mouse | 67448 | Plxdc2 | plexin domain containing 2 | XM_006497531.3 | 78.8% | 82.7% | (many diffs) |
| 5 | mouse | 67448 | Plxdc2 | plexin domain containing 2 | NM_026162.6 | 78.5% | 82.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gaggttcccg aaggccgacc tggccgctgc aggagttatg ttactttgcc 121 acttcttcac ggaccagttt cagttcgccg atgggaaacc cggagaccaa atccttgatt 181 ggcagtatgg agttactcag gccttccctc acacagagga ggaggtggaa gttgattcac 241 acgcgtacag ccacaggtgg aaaagaaact tggactttct caaggcggta gacacgaacc 301 gagcaagcgt cggccaagac tctcctgagc ccagaagctt cacagacctg ctgctggatg 361 atgggcagga caataacact cagatcgaga gagtgaatct gtccttcgat tttccatttt 421 atggccactt cctacgtgaa atcactgtgg caaccggggg tttcatatac actggagaag 481 tcgtacatcg aatgctaaca gccacacagt acatagcacc tttaatggca aatttcgatc 541 ccagtgtatc cagaaattca actgtcagat attttgataa tggcacagca cttgtggtcc 601 agtgggacca tgtacatctc caggataatt ataacctggg aagcttcaca ttccaggcaa 661 ccctgctcat ggatggacga atcatctttg gatacaaaga aattcctgtc ttggtcacac 721 agataagttc aaccaatcat ccagtgaaag tcggactgtc cgatgcattt gtcgttgtcc 781 acaggatcca acaaattccc aatgttcgaa gaagaacaat ttatgaatac caccgagtag 841 agctacaaat gtcaaaaatt accaacattt cggctgtgga gatgacccca ttacccacat 901 gcctccagtt taacagatgt ggcccctgtg tatcttctca gattggcttc aactgcagtt 961 ggtgtagtaa acttcaaaga tgttccagtg gatttgatcg tcatcggcag gactgggtgg 1021 acagtggatg ccctGAAGAG TCAAAAGAGA AGATGTGTGA GAATACAGAA CCAGTGGAAA 1081 CTTCTTCTCG AACCACCACA ACCGTAGGAG CGACAACCAC CCAGTTCAGG GTCCTAACTA 1141 CCACCAGAAG AGCAGTGACT TCTCAGTTTC CCACCAGCCT CCCTACAGAA GATGATACCA 1201 AGATAGCACT ACATCTAAAA GATAATGGAG CTTCTACAGA TGACAGTGCA GCTGAGAAGA 1261 AAGGGGGAAC CCTCCACGCT GGCCTCATCA TTGGAATCCT CATCCTGGTC CTCATTGTAG 1321 CCACAGCCAT TCTTGTGACA GTCTATATGT ATCACCACCC AACATCAGCA GCCAGCATCT 1381 TCTTTATTGA GAGACGCCCA AGCAGATGGC CTGCGATGAA GTTTAGAAGA GGCTCTGGAC 1441 ATCCTGCCTA TGCTGAAGTT GAACCAGTTG GAGAGAAAGA AGGCTTTATT GTATCAGAGC 1501 AGTGCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATTGCGCTGC GACAGTTGAT CTCACACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt