Construct: ORF TRCN0000481203
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007412.1_s317c1
- Derived from:
- ccsbBroadEn_06954
- DNA Barcode:
- CTGATTTCTGGCCTATTCTCAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC1A1 (6505)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481203
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | NM_004170.6 | 99.9% | 99.8% | 274C>G |
| 2 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_011518007.1 | 95.7% | 95.6% | 90_158del;343C>G |
| 3 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_011518008.3 | 95.6% | 92.7% | (many diffs) |
| 4 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_011518009.3 | 95.6% | 94% | (many diffs) |
| 5 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_017015043.1 | 93% | 92.9% | 274C>G;766_767ins108 |
| 6 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_011518010.1 | 90.9% | 90.8% | 89_90ins141;133C>G |
| 7 | human | 6505 | SLC1A1 | solute carrier family 1 mem... | XM_017015042.1 | 89.1% | 89% | 90_158del;343C>G;835_836ins108 |
| 8 | mouse | 20510 | Slc1a1 | solute carrier family 1 (ne... | NM_009199.2 | 85.6% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1638
- ORF length:
- 1572
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gaaaccggcg aggaaaggat gcgagtggaa gcgcttcctg aagaataact 121 gggtgttgct gtccaccgtg gccgcggtgg tgctaggcat taccacagga gtcttggttc 181 gagaacacag caacctctca actctagaga aattctactt tgcttttcct ggagaaattc 241 taatgcggat gctgaaactc atcattttgc cattaattat atccagcatg attacaggtg 301 ttgctgcact ggattccaac gtatccggaa aaattggtgt gcgcgctgtc gtgtattatt 361 tctgtaccac tctcattgct gttattctag gtattgtgct ggtggtgagc atcaagcctg 421 gtgtcaccca gaaagtgggt gaaattgcga ggacaggcag cacccctgaa gtcagtacgg 481 tggatgccat gttagatctc atcaggaata tgttccctga gaatcttgtc caggcctgtt 541 ttcagcagta caaaactaag cgtgaagaag tgaagcctcc cagcgatcca gagatgaaca 601 tgacagaaga gtccttcaca gctgtcatga caactgcaat ttccaagaac aaaacaaagg 661 aatacaaaat tgttggcatg tattcagatg gcataaacgt cctgggcttg attgtctttt 721 gccttgtctt tggacttgtc attggaaaaa tgggagaaaa gggacaaatt ctggtggatt 781 tcttcaatgc tttgagtgat gcaaccatga aaatcgttca gatcatcatg tgttatatgc 841 cactaggtat tttgttcctg attgctggga agatcataga agttgaagac tgggaaatat 901 tccgcaagct gggcctttac atggccacag tcctgactgg gcttgcaatc cactccattg 961 taattctccc gctgatatat ttcatagtcg tacgaaagaa ccctttccga tttgccatgg 1021 gaatggccca ggctctcctg acagctctca tgatctcttc cagttcagca acactgcctg 1081 tcaccttccg ctgtgctgaa gaaaataacc aggtggacaa gaggatcact cgattcgtgt 1141 tacccgttgg tgcaacaatc aacatggatg ggactgcgct ctatgaagca gtggcagcgg 1201 tgtttattgc acagttgaat gacctggact tgggCATTGG GCAGATCATC ACCATCAGTA 1261 TCACGGCCAC ATCTGCCAGC ATCGGAGCTG CTGGCGTGCC CCAGGCTGGC CTGGTGACCA 1321 TGGTGATTGT GCTGAGTGCC GTGGGCCTGC CCGCCGAGGA TGTCACCCTG ATCATTGCTG 1381 TCGACTGGCT CCTGGACCGG TTCAGGACCA TGGTCAACGT CCTTGGTGAT GCTTTTGGGA 1441 CGGGCATTGT GGAAAAGCTC TCCAAGAAGG AGCTGGAGCA GATGGATGTT TCATCTGAAG 1501 TCAACATTGT GAATCCCTTT GCCTTGGAAT CCACAATCCT TGACAACGAA GACTCAGACA 1561 CCAAGAAGTC TTATGTCAAT GGAGGCTTTG CAGTAGACAA GTCTGACACC ATCTCATTCA 1621 CCCAGACCTC ACAGTTCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTGATTT CTGGCCTATT 1801 CTCAGTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt