Construct: ORF TRCN0000481247
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011896.1_s317c1
- Derived from:
- ccsbBroadEn_05915
- DNA Barcode:
- GATCTATTCCAGGGTGACACGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8B (732)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481247
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 732 | C8B | complement C8 beta chain | NM_000066.4 | 99.9% | 99.8% | 349G>A |
| 2 | human | 732 | C8B | complement C8 beta chain | XM_017002235.1 | 99.9% | 99.8% | 349G>A |
| 3 | human | 732 | C8B | complement C8 beta chain | NM_001278543.1 | 91.2% | 91.2% | 0_1ins156 |
| 4 | human | 732 | C8B | complement C8 beta chain | NM_001278544.1 | 88.2% | 84.4% | (many diffs) |
| 5 | human | 732 | C8B | complement C8 beta chain | XR_001737397.1 | 73.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1842
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagaattcc aggacatggg cttggagggc gccggtggag ctatttcttc 121 tctgtgctgc cctgggctgt ctcagtttgc ctggctccag aggtgaaagg ccacattcct 181 ttgggtcaaa tgcagtcaac aagagctttg ctaagagcag acagatgcgg agtgtggatg 241 ttaccctgat gcccattgat tgtgagctgt ctagttggtc ctcttggacc acatgtgacc 301 cctgtcagaa gaaaaggtac aggtatgcct acttgctcca gccctctcag ttccatgggg 361 aaccgtgcaa cttctctgac aaggaagtcg aagactgtgt taccaacaga ccatgcagaa 421 gtcaagtgcg atgtgaaggc tttgtgtgtg cacagacagg aaggtgtgta aaccgcagac 481 ttctttgcaa tggggacaat gactgtggag accagtcaga tgaagcaaac tgtagaagga 541 tttataaaaa atgtcagcat gaaatggacc aatactgggg aattggcagt ctggccagtg 601 ggataaattt gttcacaaac agttttgagg gcccagttct tgatcacagg tattatgcag 661 gtggatgctc cccgcattac atcctgaaca cgaggtttag gaagccctac aatgtggaaa 721 gctacacgcc acagacccaa ggcaaatacg aattcatatt aaaagagtat gaatcatact 781 cagattttga acgcaatgtc acagagaaaa tggcaagcaa gtctggtttc agttttggtt 841 ttaaaatacc tggaatattt gaacttggca tcagtagtca aagtgatcga ggcaaacact 901 atattaggag aaccaaacga ttctctcata ctaaaagcgt atttctgcat gcacgctctg 961 accttgaagt agcacattac aagctgaaac ccagaagcct catgctccat tacgagttcc 1021 ttcagagagt taagcggctg cccctggagt acagctacgg ggaatacaga gatctcttcc 1081 gtgattttgg gacccactac atcacagagg ctgtgcttgg gggcatttat gaatacaccc 1141 tcgttatgaa caaagaggcc atggagagag gagattatac tcttaacaac gtccatgcct 1201 gtgccaaaaa tgattttaaa attggtggtg ccattgaaga ggtctacgtc agtctgggtg 1261 tgtctgtagg caaatgcaga ggtattctga atgaaataaa agacagaaac aagagggaca 1321 ccatggtgga ggacttggtg gtcctggtac gaggaggggc aagtgagcac atcaccaccc 1381 tggcatacca ggagctgccg acggcggacc TGATGCAGGA GTGGGGAGAC GCTGTGCAGT 1441 ACAACCCAGC CATCATCAAA GTTAAGGTGG AGCCTCTGTA TGAACTAGTG ACAGCCACAG 1501 ATTTTGCCTA TTCCAGCACA GTGAGGCAGA ACATGAAGCA GGCACTGGAG GAGTTCCAGA 1561 AGGAAGTTAG TTCCTGCCAC TGTGCTCCCT GCCAAGGAAA TGGAGTCCCT GTCCTGAAAG 1621 GATCACGCTG TGACTGCATC TGTCCTGTTG GATCCCAAGG CCTAGCCTGT GAGGTCTCCT 1681 ATCGGAAGAA TACCCCCATT GATGGGAAGT GGAATTGCTG GTCAAATTGG TCTTCATGCT 1741 CTGGAAGACG TAAGACAAGA CAAAGGCAGT GTAACAATCC ACCTCCTCAA AATGGGGGTA 1801 GCCCCTGTTC AGGCCCTGCT TCAGAAACAC TTGACTGCTC CTTGCCAACT TTCTTGTACA 1861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1981 AGGACGAGAT CTATTCCAGG GTGACACGGT TACGCGTTAA GTCgacaatc aacctctgga 2041 ttacaaaatt tgtgaaagat t