Construct: ORF TRCN0000481249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010316.1_s317c1
- Derived from:
- ccsbBroadEn_07409
- DNA Barcode:
- CCCATCACGTCTCGATTCCGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HS6ST1 (9394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481249
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9394 | HS6ST1 | heparan sulfate 6-O-sulfotr... | NM_004807.3 | 99.9% | 100% | 177A>G |
| 2 | mouse | 50785 | Hs6st1 | heparan sulfate 6-O-sulfotr... | NM_015818.2 | 91% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1302
- ORF length:
- 1233
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggcggcgg cgcgccggcg gcaggaccat ggttgagcgc gccagcaagt 121 tcgtgctggt ggtggcgggc tcggtgtgct tcatgctcat cttgtaccag tacgcgggcc 181 caggactgag cctgggcgcg cccggcggcc gcgcgccgcc cgacgacctg gacctgttcc 241 ccacgcccga cccccactac gagaagaagt actacttccc ggtccgcgag ctggagcgct 301 cgctgcgctt cgacatgaag ggcgacgacg tgatcgtctt cctgcacatc cagaagacgg 361 gcggcaccac cttcggccgc cacctcgtgc agaacgtacg cctcgaggtg ccgtgcgact 421 gccggcccgg ccagaagaag tgcacctgct accggcccaa ccgccgcgag acttggctct 481 tctcccgctt ctccaccggc tggagctgcg ggctgcacgc cgactggacc gagctcacca 541 actgcgtgcc cggcgtgctg gaccgccgcg actccgccgc gctgcgcacg cccaggaagt 601 tctactacat caccctgcta cgagaccccg tgtcccgcta cctgagcgag tggcggcatg 661 tgcagagggg tgccacgtgg aagacgtcgt tgcatatgtg tgatgggcgc acgcccacgc 721 ctgaggagct gccgccctgc tacgagggca cggactggtc gggctgcacg ctacaggagt 781 tcatggactg cccgtacaac ctggccaaca accgccaggt gcgcatgctg gccgacctga 841 gcctggtggg ctgctacaac ctgtccttca tccccgaggg caagcgggcc cagctgctgc 901 tcgagagcgc caagaagaac ctgcggggca tggccTTCTT CGGCCTGACC GAGTTCCAGC 961 GCAAGACGCA GTACCTGTTC GAGCGGACGT TCAACCTCAA GTTCATCCGG CCCTTCATGC 1021 AGTACAATAG CACGCGGGCG GGCGGCGTGG AGGTGGATGA AGACACCATC CGGCGCATCG 1081 AGGAGCTCAA CGACCTGGAC ATGCAGCTGT ACGACTACGC CAAGGACCTC TTCCAGCAGC 1141 GCTACCAGTA CAAGCGGCAG CTGGAGCGCA GGGAGCAGCG CCTGAGGAGC CGCGAGGAGC 1201 GTCTGCTGCA CCGGGCCAAG GAGGCACTGC CGCGGGAGGA TGCCGACGAG CCGGGCCGCG 1261 TGCCCACCGA GGACTACATG AGCCACATCA TTGAGAAGTG GTTGCCAACT TTCTTGTACA 1321 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1381 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1441 AGGACGACCC ATCACGTCTC GATTCCGTTT GACGCGTTAA GTCgacaatc aacctctgga 1501 ttacaaaatt tgtgaaagat t