Construct: ORF TRCN0000481259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002304.2_s317c1
- Derived from:
- ccsbBroadEn_02255
- DNA Barcode:
- TGGACTTACAACCAGCCCTACCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JAKMIP2 (9832)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9832 | JAKMIP2 | janus kinase and microtubul... | NM_014790.5 | 100% | 100% | |
| 2 | human | 9832 | JAKMIP2 | janus kinase and microtubul... | NM_001270941.2 | 97.7% | 97.3% | 2413_2414delTTinsAG;2416_2416delCinsAACCGAAAACA;2419_2460delinsGA |
| 3 | human | 9832 | JAKMIP2 | janus kinase and microtubul... | NM_001270934.2 | 95.2% | 94.7% | (many diffs) |
| 4 | human | 9832 | JAKMIP2 | janus kinase and microtubul... | XM_017010098.2 | 92.9% | 92.4% | (many diffs) |
| 5 | human | 9832 | JAKMIP2 | janus kinase and microtubul... | NM_001282282.2 | 92.6% | 92.1% | (many diffs) |
| 6 | mouse | 76217 | Jakmip2 | janus kinase and microtubul... | NM_001163637.1 | 88.4% | 96.6% | (many diffs) |
| 7 | mouse | 76217 | Jakmip2 | janus kinase and microtubul... | XM_006526337.3 | 87.9% | 96.2% | (many diffs) |
| 8 | mouse | 76217 | Jakmip2 | janus kinase and microtubul... | XM_011247034.2 | 85.8% | 94% | (many diffs) |
| 9 | mouse | 76217 | Jakmip2 | janus kinase and microtubul... | XM_006526338.3 | 85.3% | 93.7% | (many diffs) |
| 10 | mouse | 76217 | Jakmip2 | janus kinase and microtubul... | XM_006526340.3 | 64.9% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2496
- ORF length:
- 2430
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caagaaaggg cgaaataagg gcgagaagcc cgaggcactc attgttgccc 121 ttcaagctgc caatgaagac ctcaggacca agctcacaga cattcagata gagctgcatc 181 aagagaagtc caaggtatca aagcttgaaa gagagaagac tcaagaagcg aagaggattc 241 gtgagctgga gcagcgcaag cacacggtgc tggtgacaga actcaaagcc aagctccatg 301 aggagaagat gaaggagctg caggctgtga gggagaacct tatcaagcag cacgagcagg 361 aaatgtcaag gacggtgaag gtacgtgatg gagagatcca gaggctcaag tctgctctct 421 gtgctctccg cgacggcagc agtgacaaag taaggacagc gctcaccatt gaggcccggg 481 aggaggcccg gaaactgttt gacacagagc gccttaagct cttacaggaa attgcggacc 541 tgaaaacggc caagaagcag gtggacgagg ctctgagcaa tatgatccaa gcagataaaa 601 tcaaggctgg ggaccttcgg agtgagcatc agtcccacca agaagccatc tcgaagatca 661 agtgggagtc ggagcgggat attcggaggc tgatggatga aatcaaagcc aaggacagga 721 tcatcttttc cctggaaaag gaactggaga cccagacagg ctatgtacag aaactccaac 781 ttcagaagga ggctttggac gaacaactct ttctggtcaa ggaggctgag tgcaacatga 841 gcagcccaaa acgagaaatt ccaggaaggg caggtgatgg ttccgaacac tgcagcagtc 901 ctgatttgcg aagaaatcaa aagagaatag ctgaattgaa tgccactata agaaaattag 961 aagacaggaa taccttgctt ggagatgaac gaaatgaact gttaaaacgt gtgcgggaaa 1021 ccgaaaagca atgtaaacct ctcctggaaa ggaacaagtg cctcgccaag agaaacgatg 1081 aactgatggt gtccttgcag cgcatggaag aaaaactaaa agccgttacc aaggaaaatt 1141 cagaaatgag agaaaaaata acatcccatc cacccctgaa gaaattaaaa tctctgaatg 1201 acctcgacca agctaatgaa gaacaagaaa cagagtttct aaaacttcag gtcattgagc 1261 aacagaacat tattgatgag ctcacaaggg accgagaaaa gctcatccgt agaagaaagc 1321 atagaagaag ttccaagcca attaagaggc ctgttttgga cccgtttatt ggctatgatg 1381 aggactctat ggattcagag acatcatcca tggcctcatt tagaacagac agaacaccag 1441 ctactcctga tgatgacttg gatgaaagtt tagcagctga agaatctgaa ctaagatttc 1501 gacaattaac aaaagaatat caggccctcc aaagagcata tgccctccta caggagcaga 1561 cgggaggcat catcgacgct gaacgagaag ccaaggctca agaacagctc caagcagagg 1621 tgctaaggta taaagccaaa attgaagacc tggaagcgac tctggctcag aaagggcagg 1681 attcacactg ggtagaagat aaacaacttt tcattaagag aaaccaggag cttttagaaa 1741 agatagaaaa acaggaggca gaaaatcacc ggttacaaca agaactacag gacgccagag 1801 accagaatga gctgctggag tttcgaaacc tagagctaga agagagagag agacgatccc 1861 ctccatttaa tctccaaatt cacccattct cagatggtgt gagtgctcta cagatctact 1921 gtatgaaaga aggtgttaag gatgtgaaca tccctgatct cataaagcag cttgatatct 1981 tgggtgataa tgggaattta agaaatgaag aacaagtggc cataattcag gccagcactg 2041 tgctgtccct ggcagagaag tggatccagc agattGAAGG AGCTGAGGCT GCCCTACACC 2101 AGAAAATGAT GGAATTGGAA AGTGACATGG AACAGTTCTG CAAAATAAAA GGCTATCTGG 2161 AGGAAGAACT AGACTACAGA AAACAAGCTC TTGACCAAGC ATATATGAGA ATCCAGGAAC 2221 TAGAAGCTAC TTTGTACAAT GCTCTACAGC AAGAAACTGT TATCAAGTTT GGTGAATTAT 2281 TAAGTGAAAA ACAGCAAGAG GAGCTGAGGA CGGCAGTAGA AAAGTTACGG CGGCAAATGC 2341 TGAGGAAGAG CAGAGAGTAT GACTGTCAGA TTCTTCAGGA GAGAATGGAG CTCTTACAGC 2401 AAGCCCATCA GAGAATTCGT GACTTAGAAG ATAAAACAGA CATCCAGAAA AGACAAATAA 2461 AAGACTTAGA AGAAAAGAGT AACCGAAAAC ATGGATACCC AACTTTCTTG TACAAAGTGG 2521 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2581 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2641 ATGGACTTAC AACCAGCCCT ACCCTACGCG TTAAGTCgac aatcaacctc tggattacaa 2701 aatttgtgaa agatt