Construct: ORF TRCN0000481269
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015857.2_s317c1
- Derived from:
- ccsbBroadEn_00005
- DNA Barcode:
- TGCATTACACTAGCATGACTTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACADL (33)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481269
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 33 | ACADL | acyl-CoA dehydrogenase long... | NM_001608.4 | 100% | 100% | |
| 2 | human | 33 | ACADL | acyl-CoA dehydrogenase long... | XM_005246517.4 | 94.4% | 94.1% | (many diffs) |
| 3 | human | 33 | ACADL | acyl-CoA dehydrogenase long... | XM_017003955.1 | 67.2% | 67.2% | 0_1ins423 |
| 4 | mouse | 11363 | Acadl | acyl-Coenzyme A dehydrogena... | NM_007381.4 | 83.8% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1356
- ORF length:
- 1290
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcacgcctt ctccgagggt ccctacgcgt cctgggcggc caccgtgcgc 121 cgcgccagct gcccgccgcg cgatgttctc attccggagg ggaagaacgt ctagaaactc 181 cttctgctaa aaaattaaca gatataggaa ttcgaagaat cttttctcca gagcatgaca 241 ttttccggaa aagtgtaagg aagtttttcc aagaagaagt gattcctcat cactcagaat 301 gggagaaagc tggagaagta agtagggagg tttgggaaaa agctggaaaa caaggactgc 361 ttggtgtcaa tattgcagag catcttggtg gaattggagg ggatctgtac tccgcagcta 421 ttgtctggga ggagcaagct tattcaaatt gttcaggccc aggttttagt attcattcag 481 gtattgtcat gtcctatatt acaaaccatg gctcagaaga acagattaag cactttattc 541 cccagatgac tgcaggcaaa tgtattggtg caatagcaat gacagagcct ggagctggaa 601 gtgacttaca gggaataaaa acaaatgcta aaaaggatgg aagtgactgg attctcaatg 661 gaagcaaggt gttcatcagt aatgggtcat taagtgatgt tgtgattgta gttgcggtca 721 caaatcatga agctccctcc cctgcccatg gtattagcct ttttctggtg gaaaatggaa 781 tgaaaggatt tatcaaggga cgaaagctac ataaaatggg attaaaagcc caggataccg 841 cagaactatt ctttgaagat atacggttgc cagctagtgc cctacttGGA GAAGAGAATA 901 AAGGCTTCTA TTACATCATG AAAGAGCTTC CACAGGAAAG GCTGTTAATT GCTGATGTGG 961 CAATTTCAGC TAGTGAATTC ATGTTTGAAG AAACCAGGAA CTATGTTAAA CAAAGAAAAG 1021 CTTTTGGCAA AACAGTTGCT CACCTACAGA CAGTGCAACA TAAATTAGCA GAATTAAAAA 1081 CACATATATG TGTAACCCGA GCATTTGTGG ACAACTGTCT CCAGCTGCAT GAAGCGAAAC 1141 GTTTGGACTC CGCCACTGCT TGCATGGCGA AATATTGGGC ATCTGAGTTA CAAAATAGTG 1201 TAGCTTACGA CTGTGTACAG CTCCATGGAG GTTGGGGATA CATGTGGGAG TACCCAATTG 1261 CAAAAGCTTA TGTGGATGCC AGAGTTCAGC CAATCTATGG TGGTACAAAT GAAATAATGA 1321 AGGAGCTGAT TGCAAGAGAG ATTGTCTTTG ACAAGTACCC AACTTTCTTG TACAAAGTGG 1381 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1441 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1501 ATGCATTACA CTAGCATGAC TTAAGACGCG TTAAGTCgac aatcaacctc tggattacaa 1561 aatttgtgaa agatt