Construct: ORF TRCN0000481272
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015080.1_s317c1
- Derived from:
- ccsbBroadEn_02496
- DNA Barcode:
- GGACACCCATTCTAAATTTGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTX2 (10651)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481272
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10651 | MTX2 | metaxin 2 | NM_006554.5 | 100% | 100% | |
| 2 | human | 10651 | MTX2 | metaxin 2 | NM_001006635.3 | 95.9% | 95% | 5A>C;7A>C;8_9ins30 |
| 3 | human | 10651 | MTX2 | metaxin 2 | NM_001319097.2 | 91.2% | 91.2% | 415_416ins69 |
| 4 | human | 10651 | MTX2 | metaxin 2 | XM_011510497.2 | 81.3% | 81.3% | 0_1ins147 |
| 5 | human | 10651 | MTX2 | metaxin 2 | NM_001319098.2 | 69.4% | 68.8% | 545C>T;547_548insTAGAGGATGTAG;549_550ins228 |
| 6 | mouse | 53375 | Mtx2 | metaxin 2 | NM_016804.4 | 92.1% | 96.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 855
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tctagtggcg gaagccttcg tctcccagat tgcagctgca gaaccttggc 121 ctgaaaatgc tacattatat cagcaattga aaggggagca aattttactt tctgacaatg 181 cagcttctct tgcagtgcag gcctttttgc aaatgtgtaa cttgcctatc aaagtagttt 241 gtagggcaaa tgcagaatat atgtctccat ctggtaaagt accttttatt catgtgggaa 301 atcaagtagt atcagaactt ggtccaatag tccaatttgt taaagccaag ggccattctc 361 ttagtgatgg gctggaggaa gtccaaaaag cagaaatgaa agcttacatg gaattagtca 421 acaatatgct gttgactgca gagctgtatc ttcagtggtg tgatgaagct acagtagggg 481 agatcactca tgctaggtat ggatctcctt acccttggcc tcTGAATCAT ATTTTGGCCT 541 ATCAAAAACA GTGGGAAGTC AAACGTAAGA TGAAAGCTAT TGGATGGGGA AAGAAGACTC 601 TGGACCAGGT CTTAGAGGAT GTAGACCAGT GCTGTCAAGC TCTCTCTCAA AGACTGGGAA 661 CACAACCGTA TTTCTTCAAT AAGCAGCCTA CTGAACTTGA CGCACTGGTA TTTGGCCATC 721 TATACACCAT TCTTACCACA CAATTGACAA ATGATGAACT TTCTGAGAAG GTGAAAAACT 781 ATAGCAACCT CCTTGCTTTC TGTAGGAGAA TTGAACAGCA CTATTTTGAA GATCGTGGTA 841 AAGGCAGGCT GTCATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 901 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 961 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGACACCCAT TCTAAATTTG 1021 TTTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt