Construct: ORF TRCN0000481292
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002230.1_s317c1
- Derived from:
- ccsbBroadEn_03786
- DNA Barcode:
- TATTGACGGGACACTCAACAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPPIN (57119)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481292
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57119 | EPPIN | epididymal peptidase inhibitor | NM_020398.4 | 100% | 100% | |
| 2 | human | 57119 | EPPIN | epididymal peptidase inhibitor | NM_001302861.2 | 90.2% | 58.6% | 222_232del;381_382ins29 |
| 3 | human | 100526773 | EPPIN-WFDC6 | EPPIN-WFDC6 readthrough | NM_001198986.2 | 74.1% | 72.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 489
- ORF length:
- 399
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGATCTTC TGGACTTTTG AGCCTCCTGG 121 TGCTATTCGT CCTCTTAGCG AATGTCCAGG GACCTGGTCT GACTGATTGG TTATTTCCCA 181 GGAGATGTCC CAAAATCAGA GAAGAATGTG AATTCCAAGA AAGGGATGTG TGTACAAAGG 241 ACAGACAATG CCAGGACAAC AAGAAGTGTT GTGTCTTCAG CTGCGGAAAA AAATGTTTAG 301 ATCTCAAACA AGATGTATGC GAAATGCCAA AAGAAACTGG CCCCTGCCTG GCTTATTTTC 361 TTCATTGGTG GTATGACAAG AAAGATAATA CTTGCTCCAT GTTTGTCTAT GGTGGCTGCC 421 AGGGAAACAA TAACAACTTC CAATCCAAAG CCAACTGCCT GAACACCTGC AAGAATAAAC 481 GCTTTCCCTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATTGA CGGGACACTC AACAGTACAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt