Construct: ORF TRCN0000481293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001286.1_s317c1
- Derived from:
- ccsbBroadEn_07276
- DNA Barcode:
- TCATCTAACCTCTCCGACCAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- JRK (8629)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8629 | JRK | Jrk helix-turn-helix protein | NM_003724.4 | 99.9% | 99.8% | 1691C>T |
| 2 | human | 8629 | JRK | Jrk helix-turn-helix protein | XM_006716677.4 | 99.9% | 99.8% | 1691C>T |
| 3 | human | 8629 | JRK | Jrk helix-turn-helix protein | XM_006716678.4 | 99.9% | 99.8% | 1691C>T |
| 4 | human | 8629 | JRK | Jrk helix-turn-helix protein | NM_001077527.3 | 95.1% | 92% | (many diffs) |
| 5 | human | 8629 | JRK | Jrk helix-turn-helix protein | NM_001279352.2 | 95.1% | 92% | (many diffs) |
| 6 | human | 8629 | JRK | Jrk helix-turn-helix protein | XM_011517354.2 | 95.1% | 92% | (many diffs) |
| 7 | human | 8629 | JRK | Jrk helix-turn-helix protein | XM_011517355.2 | 93.7% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1770
- ORF length:
- 1704
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctccaagccg gctgccggga agagcagagg ggagaagcgg aagagggtgg 121 tgctgacact gaaggagaag attgacatct gcacgcgcct ggagaagggc gagagccgga 181 aggcactgat gcaggagtac aatgtgggca tgtccaccct ctacgacatc agggcccaca 241 aggcgcagct gctccggttc ttcgccagct ccgactccaa caaggcgctg gagcagcggc 301 gcacgctgca cacgcccaag ctggagcacc tggaccgcgt cctgtacgag tggttcctgg 361 ggaagcgctc cgagggcgtc cccgtgtcag gccccatgct catcgagaag gccaaggact 421 tctacgagca gatgcagctc actgagccct gcgtgttctc cggagggtgg ctttggcgct 481 ttaaggccag acacggcatt aaaaagctag atgcatccag tgaaaagcag tcagccgacc 541 accaggccgc ggagcagttc tgtgcgtttt tcaggagctt ggctgctgag cacgggctgt 601 ccgccgagca ggtttacaac gctgatgaga ccggcctttt ctggcggtgc ctgccaaatc 661 ccactccgga aggcggggct gtgcctggcc ccaagcaggg caaggaccgg ctgaccgtgc 721 tgatgtgtgc caacgccacg ggctcccaca ggctcaagcc cttggccatc gggaagtgca 781 gcggtcccag ggctttcaaa ggcatccagc acctgcccgt cgcctataag gcccagggga 841 acgcctgggt ggacaaggag attttttccg attggttcca tcatatcttt gtgccctcgg 901 tgagagagca cttcagaacc ataggtttgc cggaagacag caaagccgtt ctcttgctgg 961 acagctcccg ggctcacccg caggaggccg agctggtgtc cagtaacgtt ttcaccatct 1021 tcctgcctgc cagcgtggcc tcattggtgc agcccatgga gcagggcatt cggagagatt 1081 tcatgaggaa cttcattaac cctccggtcc ccctgcaggg cccccacgcc cgctacaaca 1141 tgaacgatgc catattcagc gtggcctgtg cctggaacgc agtccctagc cacgtcttca 1201 ggcgggcctg gaggaagctg tggccgtcgg ttgcgtttgc cgaaggctcc tcctctgagg 1261 aggagttgga ggcagagtgc ttcccagtga agccccacaa caagtccttt gcacacatcc 1321 tggagcttgt gaaggaaggc tcctcctgcc cgggCCAGCT TCGCCAGCGC CAGGCCGCCA 1381 GCTGGGGGGT AGCGGGAAGG GAGGCAGAAG GGGGACGGCC CCCTGCTGCC ACGTCGCCAG 1441 CAGAGGTTGT GTGGAGTTCA GAAAAGACTC CGAAAGCTGA CCAGGACGGC AGAGGAGATC 1501 CTGGTGAGGG CGAGGAGGTG GCCTGGGAGC AGGCGGCCGT GGCCTTTGAC GCAGTCCTGC 1561 GCTTTGCGGA GCGGCAGCCA TGCTTCAGTG CGCAGGAAGT GGGGCAGCTG CGGGCGCTGC 1621 GTGCCGTGTT CCGGAGCCAG CAGCAGGTGA GGAGGCGGCG TGGTGCCCTC GGGGCTGTGG 1681 TCAAGGTTGA AGCCCTCCAG GAGGGCCCTG GTGGCTGTGG GGCCACAGCT CAGTCTCCCT 1741 TGCCCTGCTC ATCCATAGCA GGTGACAACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1801 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1861 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCATC 1921 TAACCTCTCC GACCAATTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1981 tgaaagatt