Construct: ORF TRCN0000481295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000356.1_s317c1
- Derived from:
- ccsbBroadEn_02281
- DNA Barcode:
- ACTGGATCCCGTTGTCAACCAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RCE1 (9986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9986 | RCE1 | Ras converting CAAX endopep... | NM_005133.3 | 100% | 100% | |
| 2 | human | 9986 | RCE1 | Ras converting CAAX endopep... | NM_001032279.2 | 68.3% | 68.3% | 0_1ins312 |
| 3 | mouse | 19671 | Rce1 | Ras converting CAAX endopep... | NM_023131.1 | 89.4% | 94.8% | (many diffs) |
| 4 | mouse | 19671 | Rce1 | Ras converting CAAX endopep... | XM_006531693.3 | 61.3% | 65% | (many diffs) |
| 5 | mouse | 19671 | Rce1 | Ras converting CAAX endopep... | XM_017318083.1 | 53.8% | 36.3% | (many diffs) |
| 6 | mouse | 19671 | Rce1 | Ras converting CAAX endopep... | XM_017318084.1 | 53.8% | 36.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1056
- ORF length:
- 987
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcggcgctg ggcggggatg ggctgcgact gctgtcggtg tcgcggccgg 121 agcggccgcc cgagtcggcg gcgctgggcg gcctgggccc cgggctgtgc tgctgggtgt 181 cagtgttctc ctgcctcagc ctcgcctgct cctacgtggg cagcctctac gtctggaaga 241 gcgaactgcc cagggaccat cccgcggtca tcaagcgacg cttcaccagc gtcctggtgg 301 tgtccagtct ctcacccctg tgcgtgctgc tctggaggga actcacaggc atccagccag 361 gcacatccct gctcaccctg atgggcttca ggctggaggg cattttccca gcggcgctgc 421 tgcccctgtt gctgaccatg attcttttcc tgggcccact gatgcagctc tctatggatt 481 gcccttgtga cctggcagat gggctgaagg ttgtcctggc cccccgctcc tgggcccgct 541 gcctcacaga catgcgttgg ctgcggaaCC AAGTGATCGC CCCGCTGACA GAGGAGCTGG 601 TGTTCCGGGC CTGTATGCTG CCCATGTTAG CACCGTGCAT GGGCCTGGGC CCTGCTGTGT 661 TCACCTGCCC GCTCTTTTTT GGAGTTGCCC ATTTTCACCA TATTATTGAG CAGCTGCGTT 721 TCCGCCAGAG CAGCGTGGGG AACATCTTCT TGTCTGCTGC GTTCCAGTTC TCCTACACAG 781 CTGTCTTCGG TGCCTACACT GCTTTCCTCT TCATCCGCAC AGGACACCTG ATTGGGCCGG 841 TTCTCTGCCA TTCCTTCTGC AATTACATGG GTTTCCCAGC TGTTTGCGCG GCCTTGGAGC 901 ACCCACAGAG GCGGCCCCTG CTGGCAGGCT ATGCCCTGGG TGTGGGACTC TTCCTGCTTC 961 TGCTCCAGCC CCTCACGGAC CCCAAGCTCT ACGGCAGCCT TCCCCTTTGT GTGCTTTTGG 1021 AGCGGGCAGG GGACTCAGAG GCTCCCCTGT GCTCCTTGCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AACTGGATCC CGTTGTCAAC CAGGAACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt