Construct: ORF TRCN0000481306
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010215.1_s317c1
- Derived from:
- ccsbBroadEn_00484
- DNA Barcode:
- CTCCTAATAGGAACTCCTTGTGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFNA5 (1946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481306
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1946 | EFNA5 | ephrin A5 | NM_001962.3 | 100% | 100% | |
| 2 | human | 1946 | EFNA5 | ephrin A5 | XM_006714565.3 | 88.1% | 88.1% | 484_485ins81 |
| 3 | human | 1946 | EFNA5 | ephrin A5 | XM_011543250.3 | 87.7% | 82.6% | (many diffs) |
| 4 | human | 1946 | EFNA5 | ephrin A5 | XM_011543251.2 | 64.9% | 64.9% | 0_1ins240 |
| 5 | human | 1946 | EFNA5 | ephrin A5 | XM_017009205.1 | 64.9% | 64.9% | 0_1ins240 |
| 6 | mouse | 13640 | Efna5 | ephrin A5 | NM_207654.2 | 96.6% | 99.1% | (many diffs) |
| 7 | mouse | 13640 | Efna5 | ephrin A5 | NM_010109.3 | 84.7% | 87.2% | (many diffs) |
| 8 | mouse | 13640 | Efna5 | ephrin A5 | XM_006523598.3 | 63% | 64.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 753
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gttgcacgtg gagatgttga cgctggtgtt tctggtgctc tggatgtgtg 121 tgttcagcca ggacccgggc tccaaggccg tcgccgaccg ctacgctgtc tactggaaca 181 gcagcaaccc cagattccag aggggtgact accatattga tgtctgtatc aatgactacc 241 tggatgtttt ctgccctcac tatgaggact ccgtcccaga agataagact gagcgctatg 301 tcctcTACAT GGTGAACTTT GATGGCTACA GTGCCTGCGA CCACACTTCC AAAGGGTTCA 361 AGAGATGGGA ATGTAACCGG CCTCACTCTC CAAATGGACC GCTGAAGTTC TCTGAAAAAT 421 TCCAGCTCTT CACTCCCTTT TCTCTAGGAT TTGAATTCAG GCCAGGCCGA GAATATTTCT 481 ACATCTCCTC TGCAATCCCA GATAATGGAA GAAGGTCCTG TCTAAAGCTC AAAGTCTTTG 541 TGAGACCAAC AAATAGCTGT ATGAAAACTA TAGGTGTTCA TGATCGTGTT TTCGATGTTA 601 ACGACAAAGT AGAAAATTCA TTAGAACCAG CAGATGACAC CGTACATGAG TCAGCCGAGC 661 CATCCCGCGG CGAGAACGCG GCACAAACAC CAAGGATACC CAGCCGCCTT TTGGCAATCC 721 TACTGTTCCT CCTGGCGATG CTTTTGACAT TATTGCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 901 CCTAATAGGA ACTCCTTGTG GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt