Construct: ORF TRCN0000481307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009415.1_s317c1
- Derived from:
- ccsbBroadEn_14071
- DNA Barcode:
- TAACGCCCAATATAATTTTGCCCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPN2 (22905)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481307
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22905 | EPN2 | epsin 2 | NM_148921.3 | 99.8% | .3% | 4delA;1031T>C |
| 2 | human | 22905 | EPN2 | epsin 2 | NM_014964.4 | 91% | .3% | 4delA;596_766del;1202T>C |
| 3 | human | 22905 | EPN2 | epsin 2 | NM_001102664.1 | 60.9% | .5% | 0_1ins683;347T>C |
| 4 | mouse | 13855 | Epn2 | epsin 2 | NM_001252189.1 | 84.5% | .3% | (many diffs) |
| 5 | mouse | 13855 | Epn2 | epsin 2 | NM_010148.3 | 82.8% | .3% | (many diffs) |
| 6 | mouse | 13855 | Epn2 | epsin 2 | XM_017314253.1 | 82.8% | .3% | (many diffs) |
| 7 | mouse | 13855 | Epn2 | epsin 2 | NM_001252188.1 | 77.1% | .3% | (many diffs) |
| 8 | mouse | 13855 | Epn2 | epsin 2 | XM_006532171.3 | 77.1% | .3% | (many diffs) |
| 9 | mouse | 13855 | Epn2 | epsin 2 | XM_006532163.3 | 75.7% | .3% | (many diffs) |
| 10 | mouse | 13855 | Epn2 | epsin 2 | XM_006532165.3 | 75.7% | .3% | (many diffs) |
| 11 | mouse | 13855 | Epn2 | epsin 2 | XM_006532166.3 | 75.7% | .3% | (many diffs) |
| 12 | mouse | 13855 | Epn2 | epsin 2 | XM_006532167.3 | 75.7% | .3% | (many diffs) |
| 13 | mouse | 13855 | Epn2 | epsin 2 | XM_006532168.3 | 75.7% | .3% | (many diffs) |
| 14 | mouse | 13855 | Epn2 | epsin 2 | XM_006532169.3 | 75.7% | .3% | (many diffs) |
| 15 | mouse | 13855 | Epn2 | epsin 2 | XM_006532170.3 | 75.7% | .3% | (many diffs) |
| 16 | mouse | 13855 | Epn2 | epsin 2 | XM_011248711.2 | 75.7% | .3% | (many diffs) |
| 17 | mouse | 13855 | Epn2 | epsin 2 | XM_011248712.2 | 75.7% | .3% | (many diffs) |
| 18 | mouse | 13855 | Epn2 | epsin 2 | XM_011248713.2 | 75.7% | .3% | (many diffs) |
| 19 | mouse | 13855 | Epn2 | epsin 2 | XM_011248714.2 | 75.7% | .3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 96
- ORF length:
- 27
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgacttcgt ctatcagacg gcagatgaaa aacatcgtga acaattactc 121 agaggcagaa atcaaagtcc gggaagccac ctccaatgac ccgtggggcc cgtccagttc 181 tctgatgacc gagattgccg acctgaccta caacgtggtg gccttctcgg agatcatgag 241 catggtgtgg aagcggctga atgaccatgg caagaactgg cggcatgtgt acaaggcgct 301 gaccctgctg gactacctca tcaagacagg ctccgaacgt gtggcccagc agtgccggga 361 gaacatcttc gccatccaga ccctgaagga cttccagtac attgaccgag atggcaagga 421 ccagggcatc aatgtgcgtg agaagtcaaa gcaactggtg gctctcctca aggacgagga 481 acggttgaag gctgagaggg cccaggctct caaaaccaaa gagcgcatgg cccaggttgc 541 cactggcatg ggcagcaacc agatcacctt tgggcgaggc tccagccagc ccaacctctc 601 caccagccac tcggagcagg agtatggcaa ggccgggggc tccccggcct cctaccatgg 661 ctccacctcc ccgcgagtgt cctccgagct ggagcaagcc cggccccaga ctagtggaga 721 agaggagctt cagctgcagc tggcacttgc catgagcaga gaagtggctg agcaggaaga 781 acgcctcagg cggggtgatg acctcagatt acagatggcc ctggaagaaa gccgaaggga 841 cacagttaaa attccaaaaa agaaagagca tggctctctc ccacagcaga ctacgctgtt 901 ggatttaatg gatgctctcc ccagctcggg ccccgcggcc cagaaagcag agccctgggg 961 cccgtcagcc tccactaacc agaccaaccc ctggggcggg ccagcggctc ctgcgagtac 1021 ttcagacccc tggccatcgt ttggtaccaa gccagctgcc tccattgacc catggggggt 1081 gcccactgga gccaccgcac aatctgtccc caagaactcg gacccctggg cagcttcaca 1141 gcagcctgcc tccagtgctg ggaaaagagc ttctgacgcg tggggcgcag tctccaccac 1201 caagcccgtg tctgtctctg ggtcctttga gctcttcagt aatctgaatg gtacaattaa 1261 agatgacttt tctgaatttg acaaccttcg gacttcaaaa aaaacagccg aatctgtgac 1321 ctctctgcca tcccaaaaca atggaactac cagccctgac ccctttgagt ctcaacccct 1381 gactgtcgcc tcaagcaagc ccagcagtgc ccggaaaaca cctgagTCCT TCCTGGGCCC 1441 CAACGCGGCC CTGGTGAACC TGGACTCACT GGTGACCAGG CCTGCCCCAC CAGCCCAGTC 1501 CCTCAACCCT TTCCTGGCAC CAGGTGCTCC CGCCACCTCG GCCCCTGTTA ACCCTTTCCA 1561 GGTGAACCAG CCCCAGCCGC TGACACTGAA CCAGCTTCGG GGGAGCCCAG TCCTGGGGAC 1621 CAGCACATCC TTTGGGCCTG GCCCAGGAGT GGAGTCCATG GCTGTGGCCT CGATGACCTC 1681 CGCGGCCCCA CAGCCAGCTC TGGGGGCCAC TGGTTCCTCT CTGACACCAC TGGGCCCTGC 1741 AATGATGAAC ATGGTGGGCA GTGTGGGTAT ACCCCCATCA GCAGCCCAGG CCACTGGCAC 1801 AACCAACCCT TTCCTTCTCT TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1861 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1921 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAACG CCCAATATAA 1981 TTTTGCCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt