Construct: ORF TRCN0000481326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015492.1_s317c1
- Derived from:
- ccsbBroadEn_05343
- DNA Barcode:
- TACTACCCGCTTTAATTGCCCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POC1B (282809)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 282809 | POC1B | POC1 centriolar protein B | NM_172240.3 | 100% | 100% | |
| 2 | human | 282809 | POC1B | POC1 centriolar protein B | NM_001199777.2 | 91.2% | 91.2% | 0_1ins126 |
| 3 | human | 282809 | POC1B | POC1 centriolar protein B | NR_037659.2 | 41.4% | (many diffs) | |
| 4 | human | 282809 | POC1B | POC1 centriolar protein B | NR_037660.2 | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcagccacg gaggaccccg ttctggagcg ttatttcaaa ggccacaaag 121 ctgcgatcac ctccttggac ctcagcccca acggcaagca acttgctact gcttcttggg 181 atacctttct catgctatgg aatttcaagc cacatgctag agcttacaga tatgtgggtc 241 acaaggatgt tgtaaccagc gtgcagtttt ctccacatgg aaacttattg gcgtctgcct 301 cacgagacag aaccgtgaga ctctggattc ctgataagag aggaaaattc tcagaattta 361 aagctcatac agctccagtt cgaagtgtag acttttcagc tgatggccag tttctagcta 421 cagcttctga agacaaatcc ataaaagtat ggagcatgta tcgccagcgc ttcctgtatt 481 ccttgtatcg acatacacac tgggtacgct gtgccaaatt ttcacccgat ggaagactaa 541 ttgtgtcatg tagtgaggat aaaactatta aaatttggga taccacaaat aagcaatgtg 601 ttaataactt ctcagattcc gttggatttg caaattttgt ggactttaac cctagtggta 661 catgcatagc ttcagcaggt tctgatcaaa ctgtgaaagt ctgggatgta agagtgaaca 721 aattactaca gcattaccaa gttcacagcg gtggagttaa ttgcatatca ttccatcctt 781 cgggtaacta tctcatcaca gcttcttcag atggtaccct taagattctg gacctcttag 841 aaggaaggct catctataca cttcaaggac atacgggacc tgtctttact gtttcatttt 901 caaaaggtgg agagctattt gcatcaggag gtgcagacac acaggtctta ttatggagga 961 ctaactttga tgaattgcat tgtaaaggtc ttaccaaaag aaatctcaaa agattacatt 1021 ttgattcacc accacatctt cttgatatct acccaagaac accacatccc catgaggaaa 1081 aagttgagac tgtagaaatt aaTCCAAAGC TTGAGGTAAT CGATTTGCAG ATCTCTACTC 1141 CCCCTGTTAT GGATATCCTT TCTTTTGATT CTACCACAAC AACAGAAACC AGTGGTAGGA 1201 CTCTGCCAGA CAAGGGTGAA GAGGCCTGTG GATATTTCTT GAACCCTTCC TTAATGTCAC 1261 CAGAATGTTT GCCAACAACC ACGAAAAAGA AAACAGAAGA CATGAGTGAC CTCCCCTGTG 1321 AAAGTCAAAG GAGCATACCT CTCGCTGTGA CTGATGCTTT AGAGCATATT ATGGAACAAC 1381 TCAATGTTTT GACACAGACT GTTTCAATCT TGGAGCAGCG ACTGACTTTG ACAGAGGATA 1441 AGCTGAAAGA CTGCCTTGAA AATCAGCAAA AGCTTTTCAG TGCTGTCCAA CAGAAAAGCT 1501 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGATACTA CCCGCTTTAA TTGCCCTGTA CGCGTTAAGT 1681 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt