Construct: ORF TRCN0000481330
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013451.2_s317c1
- Derived from:
- ccsbBroadEn_07315
- DNA Barcode:
- ACATTGTCCTTTTTTCCTCAGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALDH1A2 (8854)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481330
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8854 | ALDH1A2 | aldehyde dehydrogenase 1 fa... | NM_170696.3 | 99.9% | 99.7% | 16A>C |
| 2 | human | 8854 | ALDH1A2 | aldehyde dehydrogenase 1 fa... | NM_003888.4 | 92.5% | 92.4% | 16A>C;682_795del |
| 3 | human | 8854 | ALDH1A2 | aldehyde dehydrogenase 1 fa... | NM_001206897.1 | 87.3% | 86.1% | (many diffs) |
| 4 | human | 8854 | ALDH1A2 | aldehyde dehydrogenase 1 fa... | NM_170697.3 | 74.1% | 74.1% | 0_1ins288;394_507del |
| 5 | mouse | 19378 | Aldh1a2 | aldehyde dehydrogenase fami... | NM_009022.4 | 83.5% | 89.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1506
- ORF length:
- 1440
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ttccagcaag ctagagatgc ccggcgaggt gaaggccgac cccgccgccc 121 tcatggcgtc gctgcacctc ctgccgtcgc ccacgcccaa tctcgaaatt aagtacacca 181 agatctttat aaacaacgag tggcagaact cagagagtgg gagagtgttc cctgtctata 241 atccagccac aggagaacag gtgtgtgaag ttcaagaagc agacaaggca gatatagaca 301 aagcagtgca ggcagcccgc ctggctttct ctcttggttc agtgtggaga aggatggatg 361 cttcagaaag gggacgtctg ttggataagc ttgcagactt ggtggaacgg gacagggcag 421 ttcttgcaac catggaatcc ctaaatggtg gcaaaccatt cctgcaagct ttttatgtgg 481 atttgcaggg cgtcatcaaa acctttcgat attacgcagg ctgggctgat aaaattcatg 541 ggatgaccat tcctgtagat ggagactatt ttacctttac aagacatgaa cccattggag 601 tgtgtggaca gatcatccca tggaacttcc ccctgctgat gtttgcctgg aaaatagctc 661 cagctttgtg ctgtggcaat acagtagtta ttaagccagc agagcaaaca ccactcagtg 721 cactctacat gggagccctc atcaaggagg ttggaaagct tatccaagaa gcagctggaa 781 gaagtaattt gaagagagta actctggaac ttggaggcaa aagtcctaat attatttttg 841 ctgatgctga cttggactat gctgtggagc aggcccacca gggtgtgttc ttcaatcaag 901 gtcagtgctg cactgcaggc tctcgcatct tcgtggagga gtccatctat gaggagtttg 961 tgagaagaag cgtggagcgg gccaagaggc gcgtagtggg gagtcccttt gaccccacca 1021 ctgagcaggg tccccagatt gataagaaac agtacaacaa gaTCTTGGAA CTCATCCAGA 1081 GTGGTGTGGC TGAGGGCGCC AAGCTGGAAT GTGGAGGCAA AGGACTGGGC CGAAAGGGGT 1141 TTTTCATTGA GCCCACAGTG TTTTCCAACG TCACTGATGA TATGCGGATT GCCAAGGAGG 1201 AGATCTTTGG CCCTGTTCAG GAAATTTTGA GATTTAAGAC GATGGATGAA GTTATCGAAA 1261 GAGCCAATAA CTCAGACTTT GGACTCGTAG CAGCTGTCTT TACTAATGAC ATCAACAAGG 1321 CCCTCACAGT GTCTTCTGCA ATGCAAGCTG GGACTGTTTG GATCAATTGT TACAATGCCT 1381 TAAATGCCCA GAGCCCCTTT GGGGGATTCA AGATGTCTGG AAATGGGAGA GAAATGGGAG 1441 AATTTGGCTT GCGGGAGTAC TCAGAAGTTA AGACGGTGAC AGTAAAGATC CCCCAGAAGA 1501 ACTCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1561 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1621 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACATTGTCC TTTTTTCCTC AGGTTACGCG 1681 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt