Construct: ORF TRCN0000481332

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012828.1_s317c1
Derived from:
ccsbBroadEn_04197
DNA Barcode:
ATTAGGTCAAATGGACCTCCCGCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFHC2 (80258)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481332
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80258 EFHC2 EF-hand domain containing 2 NM_025184.4 100% 100%
2 human 80258 EFHC2 EF-hand domain containing 2 XM_024452453.1 95.7% 95.7% 0_1ins96
3 human 80258 EFHC2 EF-hand domain containing 2 XM_006724562.2 73.8% 73.8% 0_1ins588
4 human 80258 EFHC2 EF-hand domain containing 2 XR_949019.1 46.1% 1_77del;2023_2024delAA;2327_4870del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2313
ORF length:
2247
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc cctgcctctg ctgccgggca acagcttcaa ccgcaacgtg ggaaaggaga
121agtttcacaa atcccaacat tggggctttt gcaacaatgt tatgatgttg gtgagtgatg
181aaaagcctgg aataggtgga gaaccacttc tggggcaaaa gataaagcct aaatgtagca
241tatatcctaa aggagatgga agtgatgtac catcatgggt agcctttgat aaacaggtat
301tatcttttga tgcctatttg gaagaggaag tacttgataa aagccaaacc aactacagaa
361taagatacta taaaatctac ttctaccctg aagatgacac aattcaagta aatgaaccag
421aggtgaaaaa tagtggatta cttcaaggga cttctatccg gcgtcatcgg attactcttc
481cgcctcctga tgaggatcag ttttatactg tgtatcattt taatgtcggc acagaggttg
541tcttctatgg ccggacattc aagatttatg actgtgatgc attcacaaga aactttttga
601ggaaaatagg ggtcaaagtg aatcccccag tgcaatgtcc agaagatcct tacatgaaga
661ttcggagaga ggttgtagaa cacgtagagc ccttacgtcc ctacgaatcc ctcgacaccc
721tgaaacagtt cctccagtat catggcaaga ttttgtgttt cttctgcctg tgggatgact
781cagtctcaat gtttggagac cgtagagaac tcatcctgca ttacttcttg tgtgatgata
841ctattgaaat caaagaattg cttccacaca gctcaggccg agatgctcta aaaatgttcc
901tccggaggag taagctaccc aagaattgcc cacctagagt ctatcaacca ggccagataa
961cagatcgagc agttctcaat tcatatggtg actttataaa gaaccaagcg gatggctacc
1021tgttcgatag atataagcta ggaaaagtag accaagagtt ttacaaagat agtgacctgt
1081ccctaggagt caccatcaat gtgtggggaa gaaaagtgct cctttatgac tgtgatgaat
1141ttacgaagtc ttattataag tctaaatatg gaattgagaa ctttacctca gtttcatgca
1201agcctccttc tcctcctcca aaaatagaaa ggaaatttcc accttacaac ggttttggtt
1261ctgaagagga ttctctccgt aactgcatag acctcaagcc cacacctcat cggaggaact
1321tcaagaagtt tatggaaaaa gacagctatg gctccaaaag caatatactc cgtttttttg
1381caaaactagt cacagacaaa tgtgttgact tggacaggat gtttgttatt tcatattatc
1441tcggtgatga caccatttca gtgtttgaac ctatagagag gaattcagga attgctggtg
1501ggatgttctt gaaaagaagt cgcgttaaga agcctggaca agaagtcttt aaaagtgaac
1561tatctgaata tatcaaggcc gaggagctgt acattggagt cacggtgaat gtgaatggtt
1621acctatttcg tttgctcaat gctgatgagt ataccttaaa ctacatggag cagaatacag
1681ataagtatcc tttcagtaac ctcaaacttg ccctacaaaa gctgaagcaa gaagaaggaa
1741aatccagaga gctcaagcag gtatttaaag ctgctgactc taagcacaca aatatggtgg
1801attataatac attcagagac atattgatgt ctttgactgt tggaaacctt gcagagcaag
1861aatttgtaac cattgcacgt cactaccgtg tgcctgaggG CACATGTTCA GATATGGATT
1921TCTTAATCGC ACTGGCCCAC GAAAAGTTCA AGAAAAATAT GTTTGAGAAT TTCGACACTT
1981TCATTTATTC CTGTGTGTAT GAAGATCGAG AAAAAAAAAA TGTATTACCC ACCAAAGACA
2041TTAAAAGGCT GTGCAAATCC TCCAGATTAC CTTTGAGTGA TGATCTTCTA GAATCCTTAT
2101TGTCAAGGTT TGAAGACAGT GAAAAACAAA TAGATTATAA GTCATTTTTC TCTGCCCTGA
2161ACTGGAGAAA GAATCCAGTG CCTGAATTGC AACCAGCATC ATACCTTAAA GAGAGATGTG
2221AAGATGTTTG GCTTGGTATG CCATCACCTA TTCCTGCGAA ATACATTGAC TACTGGACCT
2281TTTTGAAGGA CGCGTTTGGC TTAGAGGAGG AATACCCAAC TTTCTTGTAC AAAGTGGTTG
2341ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
2401GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT
2461TAGGTCAAAT GGACCTCCCG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat
2521ttgtgaaaga tt

Download FASTA (ORF) (Full)