Construct: ORF TRCN0000481332
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012828.1_s317c1
- Derived from:
- ccsbBroadEn_04197
- DNA Barcode:
- ATTAGGTCAAATGGACCTCCCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFHC2 (80258)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481332
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80258 | EFHC2 | EF-hand domain containing 2 | NM_025184.4 | 100% | 100% | |
| 2 | human | 80258 | EFHC2 | EF-hand domain containing 2 | XM_024452453.1 | 95.7% | 95.7% | 0_1ins96 |
| 3 | human | 80258 | EFHC2 | EF-hand domain containing 2 | XM_006724562.2 | 73.8% | 73.8% | 0_1ins588 |
| 4 | human | 80258 | EFHC2 | EF-hand domain containing 2 | XR_949019.1 | 46.1% | 1_77del;2023_2024delAA;2327_4870del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2313
- ORF length:
- 2247
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cctgcctctg ctgccgggca acagcttcaa ccgcaacgtg ggaaaggaga 121 agtttcacaa atcccaacat tggggctttt gcaacaatgt tatgatgttg gtgagtgatg 181 aaaagcctgg aataggtgga gaaccacttc tggggcaaaa gataaagcct aaatgtagca 241 tatatcctaa aggagatgga agtgatgtac catcatgggt agcctttgat aaacaggtat 301 tatcttttga tgcctatttg gaagaggaag tacttgataa aagccaaacc aactacagaa 361 taagatacta taaaatctac ttctaccctg aagatgacac aattcaagta aatgaaccag 421 aggtgaaaaa tagtggatta cttcaaggga cttctatccg gcgtcatcgg attactcttc 481 cgcctcctga tgaggatcag ttttatactg tgtatcattt taatgtcggc acagaggttg 541 tcttctatgg ccggacattc aagatttatg actgtgatgc attcacaaga aactttttga 601 ggaaaatagg ggtcaaagtg aatcccccag tgcaatgtcc agaagatcct tacatgaaga 661 ttcggagaga ggttgtagaa cacgtagagc ccttacgtcc ctacgaatcc ctcgacaccc 721 tgaaacagtt cctccagtat catggcaaga ttttgtgttt cttctgcctg tgggatgact 781 cagtctcaat gtttggagac cgtagagaac tcatcctgca ttacttcttg tgtgatgata 841 ctattgaaat caaagaattg cttccacaca gctcaggccg agatgctcta aaaatgttcc 901 tccggaggag taagctaccc aagaattgcc cacctagagt ctatcaacca ggccagataa 961 cagatcgagc agttctcaat tcatatggtg actttataaa gaaccaagcg gatggctacc 1021 tgttcgatag atataagcta ggaaaagtag accaagagtt ttacaaagat agtgacctgt 1081 ccctaggagt caccatcaat gtgtggggaa gaaaagtgct cctttatgac tgtgatgaat 1141 ttacgaagtc ttattataag tctaaatatg gaattgagaa ctttacctca gtttcatgca 1201 agcctccttc tcctcctcca aaaatagaaa ggaaatttcc accttacaac ggttttggtt 1261 ctgaagagga ttctctccgt aactgcatag acctcaagcc cacacctcat cggaggaact 1321 tcaagaagtt tatggaaaaa gacagctatg gctccaaaag caatatactc cgtttttttg 1381 caaaactagt cacagacaaa tgtgttgact tggacaggat gtttgttatt tcatattatc 1441 tcggtgatga caccatttca gtgtttgaac ctatagagag gaattcagga attgctggtg 1501 ggatgttctt gaaaagaagt cgcgttaaga agcctggaca agaagtcttt aaaagtgaac 1561 tatctgaata tatcaaggcc gaggagctgt acattggagt cacggtgaat gtgaatggtt 1621 acctatttcg tttgctcaat gctgatgagt ataccttaaa ctacatggag cagaatacag 1681 ataagtatcc tttcagtaac ctcaaacttg ccctacaaaa gctgaagcaa gaagaaggaa 1741 aatccagaga gctcaagcag gtatttaaag ctgctgactc taagcacaca aatatggtgg 1801 attataatac attcagagac atattgatgt ctttgactgt tggaaacctt gcagagcaag 1861 aatttgtaac cattgcacgt cactaccgtg tgcctgaggG CACATGTTCA GATATGGATT 1921 TCTTAATCGC ACTGGCCCAC GAAAAGTTCA AGAAAAATAT GTTTGAGAAT TTCGACACTT 1981 TCATTTATTC CTGTGTGTAT GAAGATCGAG AAAAAAAAAA TGTATTACCC ACCAAAGACA 2041 TTAAAAGGCT GTGCAAATCC TCCAGATTAC CTTTGAGTGA TGATCTTCTA GAATCCTTAT 2101 TGTCAAGGTT TGAAGACAGT GAAAAACAAA TAGATTATAA GTCATTTTTC TCTGCCCTGA 2161 ACTGGAGAAA GAATCCAGTG CCTGAATTGC AACCAGCATC ATACCTTAAA GAGAGATGTG 2221 AAGATGTTTG GCTTGGTATG CCATCACCTA TTCCTGCGAA ATACATTGAC TACTGGACCT 2281 TTTTGAAGGA CGCGTTTGGC TTAGAGGAGG AATACCCAAC TTTCTTGTAC AAAGTGGTTG 2341 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2401 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 2461 TAGGTCAAAT GGACCTCCCG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2521 ttgtgaaaga tt